Examples of 'mm phosphate buffer' in a sentence
Meaning of "mm phosphate buffer"
mm phosphate buffer - a solution commonly used in biological and biochemical research to maintain a stable pH level. It consists of a specific ratio of phosphate salts and is used to mimic physiological conditions for experiments
How to use "mm phosphate buffer" in a sentence
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mm phosphate buffer
Comminute and suspend in a small volume of sterile distilled water or 50 mM phosphate buffer.
A 50 mM phosphate buffer containing 5 mM NaCl was used as the electrolyte.
Even more preferably, a pharmaceutical composition of the present invention comprises about 5 mM phosphate buffer.
Said rinse solution is 10 mM phosphate buffer saline, physiological saline or others.
A currently most preferred aqueous carder is 10 mM phosphate buffer.
Containers with 100 mM phosphate buffer and deionized water were loaded onto the Hamilton pipettor.
The dialysis is carried out in a 1 mM phosphate buffer at pH 7.
Crude laccase protein is isolated from conidiating A. niger mycelia in 20 mM phosphate buffer.
The mobile phase A contains a 5 mM phosphate buffer of pH 4.9 and the mobile phase B contains acetonitrile.
A currently most preferred aqueous carrier is 10 mM phosphate buffer.
The anode contained a 50 mM phosphate buffer with 50 mM KCl as an anolyte.
Suspend in a small volume of sterile distilled water or 50 mM phosphate buffer.
The artificial urine is made in 10 mM phosphate buffer and adjusted to pH 6.0.
Tyrosol was purchased from TCI Europe ( Belgium ) and stocks were prepared in 50 mM phosphate buffer.
Dialysis against the 125 mM phosphate buffer of pH 7 is then continued as before.
See also
In one embodiment, the composition comprises about 10 mM phosphate buffer.
Erythrocytes were washed extensively in 20 mM phosphate buffer ( pH 7.4 ) to remove external and intracellular glucose.
The IV bolus formulation for prodrug was in 25 mM phosphate buffer.
Then, the column was washed with 5 mM phosphate buffer for 15 minutes.
The elution was carried out by forming a concentration gradient from 50 mM to 300 mM phosphate buffer.
Said rinse solution may be 10 mM phosphate buffer saline ( PBS ) or other.
The volume was kept at approximately 200 mL with constant addition of 10 mM phosphate buffer.
The preferred buffer is 10 mM phosphate buffer.
Active fractions were pooled and dialyzed against 50 mM phosphate buffer ( pH 8 ).
The reaction mix was dialyzed against 50 mM phosphate buffer pH 6.6 over night.
For example, the elution buffer may contain about 2 mM to about 50 mM phosphate buffer.
The temperature was maintained at 22 ° C, and a 25 mM phosphate buffer was added to each solution.
After decapitation, the organs were immediately removed and homogenised in 5 mM phosphate buffer of pH 7.8.
Ellman 's reagent is added to a solution of antibody in 40 mM phosphate buffer containing 1 mM EDTA.
Biotin in excess has been later removed by dialysis against 50 mM phosphate buffer pH 7.5.
All assays were performed in 5 to 20 mM phosphate buffer.
The eluate is evaporated and the residue is resuspended in 50 mM phosphate buffer at pH 6.8.
Bound protein is eluted with a linear gradient of NaCl in 20 mM phosphate buffer pH 6.5.
First we looked for the optimal protein concentration, using the 10 mM phosphate buffer pH 6.0.
THF was evaporated and reaction mixture was diluted with 5.0 mL of 7 mM phosphate buffer.
Figure 3 shows LON-assisted aqueous solubilization of Paclitaxel in 1 mM phosphate buffer and 100 mM NaCl.
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