Examples of 'mutagenic primer' in a sentence
Meaning of "mutagenic primer"
mutagenic primer: In genetics, a mutagenic primer is a short piece of nucleic acid that is used to induce mutations in DNA during laboratory experiments. It is designed to specifically target and alter certain nucleotide sequences
How to use "mutagenic primer" in a sentence
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mutagenic primer
Sets of mutagenic primer pairs are synthesized encompassing the regions to be altered.
Each individual mutagenesis reaction contains one and only one mutagenic primer.
Sets of mutagenic primer pairs were synthezised encompassing the regions to be altered.
There would typically be minimum regions of complementarity at the ends of the mutagenic primer.
A single mutagenic primer containing both of these domains could also be used.
The terms separated and isolated are used interchangeably with respect to the single-stranded mutagenic primer.
Design a mutagenic primer that will randomly generate all possible amino acid codons.
Then plaques are picked, phages isolated and then screened with ³ ² P-labelled mutagenic primer I.
The size of the mutagenic primer may depend upon the size of the parent molecule.
The exchanged nucleotide 45 of the mutagenic primer is marked by *.
The mutagenic primer were used in a PCR reaction with a suitable opposite primer.
In contrast, the control with no mutagenic primer and no enzyme produced - 10 transformants.
A mutagenic primer ( FL64 ) containing the modified sequence was synthesized and phosphorylated.
By way of example, a molar ratio of 3:1 mutagenic primer to template may be used.
The mutagenic primer and the M13 sequencing primer can be chemically synthesized ( supra ).
See also
The second reaction used the 3 ' external primer and the sense mutagenic primer covering codon 203.
A nucleotide sequence of the mutagenic primer used for site-directed mutagenesis was as follows.
Mutagenic primer sequences for construction of 3 ' - UTR deletions Primer name.
The first reactions used the 5 ' external primer and the antisense mutagenic primer covering codon 203.
Subsequently, the resulting mutagenic primer was used in a PCR reaction with a suitable opposite primer.
Thirty-six plaques are picked, and phages screened with ³ ² P-labelled mutagenic primer II.
Subsequently, the resulting mutagenic primer may be used in a PCR reaction with a suitable opposite primer.
PCR1 used the 5 ' ( forward ) flanking primer and the reverse mutagenic primer.
Figure 1d shows a mutagenic primer for producing VH or VI variants.
The BglII site was introduced by in vitro mutagenesis using the mutagenic primer 5 ' -CAAAAGATCTTGAACAAAG-3 '.
Phosphorylation of mutagenic primer The following were added to an Eppendorf,.
Fig . 6 shows the sequences of the RNA2:VSV-G mutagenic primer and the hybridization probe.
Thus, in step ( c ) the mutagenic primer may be annealed to multiple different parent molecules.
Annealing of mutagenic primer to template at a ratio of 3:1.
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Examples of using Primer
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The primer includes the following modules
It was mostly gray primer and fender fill
A primer is not necessary for small repairs
Examples of using Mutagenic
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Teriflunomide was not mutagenic in vitro or clastogenic in vivo
No mutagenic or clastogenic effects were demonstrated in preclinical studies
Vandetanib has shown no mutagenic or clastogenic potential
