Examples of 'nucleoside triphosphates' in a sentence
Meaning of "nucleoside triphosphates"
nucleoside triphosphates: Refers to organic molecules that serve as energy carriers in cells, playing a crucial role in various biological processes such as DNA replication and protein synthesis. This phrase is commonly used in biochemistry and molecular biology contexts
How to use "nucleoside triphosphates" in a sentence
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nucleoside triphosphates
The concentration of nucleoside triphosphates can vary widely.
Nucleoside triphosphates and their analogs.
The activity of nevirapine does not compete with template or nucleoside triphosphates.
Appropriate nucleoside triphosphates will also generally be required.
Polymerases are enzymes that synthesize polynucleotide chains from nucleoside triphosphates.
Nucleoside triphosphates are commercially available.
The kit can also include nucleoside triphosphates and a nucleotide polymerase.
Nucleoside triphosphates with such sugar units can also be useful for DNA sequencing.
It can also act on ADP and other nucleoside triphosphates and diphosphates.
Thus, nucleoside triphosphates are a type of nucleotide.
Optionally, the reagents are fluorescently labeled nucleoside triphosphates.
One of the nucleoside triphosphates was labeled with radioactive phosphorus.
In addition, glycosylases do not efficiently recognize nucleoside triphosphates as substrates.
Radiolabeled nucleoside triphosphates are preferably used for this purpose.
The system according to claim 5, wherein said reagents are fluorescently labeled nucleoside triphosphates.
See also
Similarly any convenient nucleoside triphosphates for conventional base pairing may be used.
To show complete blocking, a chase is performed with the four native, unblocked nucleoside triphosphates.
Solutions containing the normal nucleoside triphosphates are also prepared,.
Nucleoside triphosphates can not be absorbed well, so all nucleoside triphosphates are typically made de novo.
In addition to polymerase, nucleoside triphosphates are added, preferably all four bases.
The only limitation is the amount of the primers, thermostable enzyme, and nucleoside triphosphates present.
These modified nucleoside triphosphates are also termed herein as ( nucleotide ) analogs.
When used in the extension / elongation context, the nucleoside-containing reactant comprises nucleoside triphosphates or analogs thereof.
Preferred nucleoside triphosphates are the deoxyribonucleoside triphosphates dATP, dCTP, dGTP and dTTP collectively termed dNTPs.
Adding a completion reagent comprising a mixture of nucleoside triphosphates to the partially polymerized sample ;.
PCR utilizes a nucleic acid polymerase, or enzyme that catalyzes the polymerization of nucleoside triphosphates.
Nucleoside triphosphates -- a nucleoside having a 5 ' triphosphate substituent, preferably a deoxynucleoside triphosphate.
In the present invention 1 to 3 different nucleoside triphosphates are employed.
Nucleoside triphosphates -- nucleosides having a 5 ' - triphosphate substituent.
These include enzymes, aqueous buffers, salts and nucleoside triphosphates.
In a preferred embodiment, the nucleoside triphosphates are dATP, dCTP, dGTP and dTTP.
Optionally, at least two oligonucleotide primers ; and optionally, at least four different nucleoside triphosphates.
The method of claim 10 wherein said chain-terminating nucleoside triphosphates are dideoxynucleoside triphosphates.
These include enzymes, aqueous buffers, salts, amplification primers, target nucleic acid and nucleoside triphosphates.
The method of claim 10 wherein said nucleoside triphosphates are dATP, dCTP, dGTP and dTTP.
These include enzymes, aqueous buffers, salts, target nucleic acid, and nucleoside triphosphates.
Mixtures of nucleoside triphosphates or 2 ' - deoxynucleotide triphosphates and 2 ', 3 ' - dideoxynucleoside triphosphates may also be used.
A test according to any one of claims 1-4, wherein the nucleoside triphosphates used are labeled.
These include enzymes, aqueous buffers, salts, oligonucleotide primers, target nucleic acid, and nucleoside triphosphates.
The method of claim 30 wherein said chain-terminating nucleoside triphosphates are labeled with fluorescent dyes.
The levels of nucleoside triphosphates ( NTP ) ;.
The method includes combining in a medium a polymerase, nucleoside triphosphates and primers P2, P4 and P5.
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Examples of using Nucleoside
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Nucleoside analogue reverse transcriptase inhibitors NRTIs
Cladribine is a purine nucleoside analogue acting as an antimetabolite
Nucleoside analogues are also used as antiviral agents
Examples of using Triphosphates
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The nucleotide triphosphates are detected using nanopores
A nanopore through which the nucleotide triphosphates pass
Appropriate nucleoside triphosphates will also generally be required
