Examples of 'pfu polymerase' in a sentence
Meaning of "pfu polymerase"
Pfu polymerase - It is a DNA polymerase enzyme that is commonly used in molecular biology for the polymerase chain reaction (PCR) technique. It is known for its high fidelity and thermostability, making it useful for various DNA amplification applications
How to use "pfu polymerase" in a sentence
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pfu polymerase
Pfu polymerase was isolated from Pyrococcus furiosus.
All fragments were generated using Pfu polymerase according to the following protocol.
With Pfu polymerase and the appropriate buffer and nucleotides.
These oligomers were used in place of template DNA in a PCR reaction with Pfu polymerase.
Pfu polymerase was obtained from Stratagene.
RIP sequences were amplified with Pfu polymerase.
All PCRs were carried out using Pfu polymerase in order to achieve greater . fidelity.
These were annealed and a double strand was formed using Pfu polymerase ( Stratagene ).
PCR was performed with Pfu polymerase ( Stratagene ) to reduce accumulation of errors.
To slow this process down, smaller amounts of Pfu polymerase were used.
Pfu polymerase ( Strategene ) was used for increased fidelity during PCR synthesis.
This was accomplished by PCR using exo Pfu polymerase obtained from Strategene.
Pfu polymerase ( Stratagene ) was used in the PCR reactions according to the manufacturer 's protocol.
Two sets of PCR were performed with Pfu polymerase and pCISMNx-wt as template.
This procedure was repeated for 18 cycles using pfu polymerase.
See also
Example 3, Cloning of codon optimized KOD and Pfu polymerase I sequences into expression vector pKBexp.
The Pfu polymerase was not added until the reaction mixture had reached 94 °C.
Both PCR steps were performed by the use of cloned Pfu polymerase AD ( Agilent Technologies ).
Example 1, Cloning of Pfu polymerase open reading frame into pASK.
PCR was performed for 35 cycles using Pfu polymerase.
PCR was performed using Pfu polymerase ( Perkin Elmer ) as follows.
Care was taken to use, when amplifying, a high fidelity ( Promega ) Pfu polymerase.
PCR amplification used pfu polymerase in pfu buffer2 ( Stratagene, La Jolla, CA ).
Amplfication was with the high-fidelity heat-stable native Pfu polymerase ( Stratagene, USA ).
Amplification was realized using Pfu polymerase ( Stratagene ).
For the amplification of a fragment of a gene of interest, Pfu polymerase ( Stratagene, USA ) was used.
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Examples of using Polymerase
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Quantitative real time polymerase chain reaction
Polymerase chain reaction testing is done upon request
Preferably the polymerase chain reaction is used
