Examples of 'pgem-t' in a sentence
Meaning of "pgem-t"
PGEM-T does not appear to have a standard definition in English
How to use "pgem-t" in a sentence
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pgem-t
Initially, these genes were cloned into pgem-t easy vector to multiply and sequencing.
The digested heavy and light chains are then respectively cloned in the sequencing vector pGEM-T.
The subtracted cDNA library was subcloned into a pGEM-T vector for further analysis.
The resulting amplification product was gel-purified and cloned into pGEM-T.
The PCR product was cloned into pGem-T intermediate plasmid and sequenced.
Clone the deleted gene loci PCR products into pGEM-T Easy.
The PCR product is cloned into pGEM-T and the sequence verified.
PCR product from amplification of orf3, cloned into pGEM-T easy.
The resulting PCR product was cloned into pGEM-T and the nucleotide sequence verified.
For example, pGEM-T Easy ( produced by Progema Corp. ) and the like are used widely.
Two fragments in the promoter region were isolated and cloned into pGEM-T vector, respectively.
The PCR product from was inserted into pGEM-T Easy vector and the nucleotide sequence confirmed.
The fragment product of the PCR reaction was bound to the pGEM-T vector ( Promega ).
The fragment was cloned into the pGEM-T Easy Vector System for DNA sequence determination.
The resulting PCR products were cloned into pGEM-T and sequenced.
See also
The fragments were cloned into the pGEM-T Easy Vector System for DNA sequence determination.
After the PCR reaction, DNA products were cloned into the pGEM-T Easy plasmid.
These were cloned into pGEM-T and subcloned to create pRTBN6.
Following A-tailing, the amplified cassettes were ligated into pGEM-T easy ( Promega ).
A positive isolate, designated pGEM-T clyA, was chosen for further use.
The obtained EPO cDNA was cloned into a cloning vector pGEM-T ( Promega Corp. ).
It is then cloned into pGEM-T easy, according to the supplier 's instructions, and then sequenced.
TrlphoA mutagenesis was carried out after electroporation of pGEM-T clyA, expressing functional S.
The sequence of a pGEM-T OPG clone is shown in Figure 6.
Those bands showing positive hybridization were subsequently cloned in pGEM-T vector ( Promega ) and sequenced.
The amplifications were cloned in a pGEM-T system ( Promega ) and selected clones were automatically sequenced.
The resultant gDNA products were gel purified, cloned into pGEM-T ( Promega ) and sequenced.
The PCR products were cloned into pGEM-T Easy ( Promega ) and a correct clone identified by sequencing.
The amplified DNA was gel purified and ligated to a pGEM-T vector ( Promega Corp. ).
Eluted DNA was cloned into the pGEM-T vector ( Promega, Madison, WI ) using standard procedures.
The Hind III / BamH I fragment was initially subcloned into pGEM-T vector ( Promega ).
The subtractive library was cloned in the pGEM-T Easy vector ( Promega ) according to the instructions.
The PCR fragment was then cloned into pGEM-T vector ( Promega ).
The products were cloned in pGEM-T and sequenced as described in example 3.2.
The fragment was ligated into the pGEM-T vector ( Promega ).
The fragment was cloned in the vector pGEM-T Easy ( Promega ) and 32 individual clones were analyzed.
The PCR fragments were cloned into pGEM-T vector ( Promega ).
Fragment C was cloned into the pGEM-T Easy vector ( Promega ), resulting in pGEM-C.
The resulting PCR products were cloned into pGEM-T and the nucleotide sequence verified.
PCR fragments were cloned in pGEM-T vector from pGEM-T Vector Systems ( Promega ).
The PCR product was ligated to the vector pGEM-T via “ T-overhang ” ligation.
The elongated fragments were cloned in pGEM-T ( Promega, the Netherlands ) and sequenced.
The PCR fragment was subsequently cloned in the pGEM-T vector ( Promega, Madison, WI, US ).
The fragments were subsequently subcloned into pGEM-T ( Invitrogen ) containing the full length IRES sequence.
The resulting product was then cloned into a pGEM-T vector ( Promega ) and sequenced.
The PCR products were ligated in a pGEM-T Vector System ( Promega ) and sequenced.
The adenosine tailed MASP-2A was then cloned into the pGEM-T easy vector, transformed into E. coli.
The PCR fragment was cloned into the pGEM-T vector ( Promega, Madison WI ) and sequenced.
The DNA fragment is cloned using pGEM-T Easy vector ( Promega ).
This fragment was cloned into pGEM-T vector ( Promega ) to obtain pGEM-ndh.
