Examples of 'phagemid' in a sentence
Meaning of "phagemid"
Phagemid likely refers to a hybrid genetic vector used in molecular biology that combines features of a bacteriophage and a plasmid.
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- A phage whose genome contains a plasmid that can be removed during the infection of a host with a second, "helper" phage.
How to use "phagemid" in a sentence
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phagemid
Phagemid preparations are prepared as recommended by the manufacturer.
This constitutes the primary phagemid library.
The phagemid was not affected by this treatment.
A protease cleavable phagemid is also constructed.
Phagemid particles were prepared as described above.
An asterisk denotes a contaminating phagemid from another library.
A phagemid is an example of such an infectious particle.
In preferred embodiments the display vector is a phagemid vector.
The phagemid particles remaining on the surface are subsequently eluted.
These recombinant vectors can also be cosmid or phagemid derivatives.
The phagemid can be harvested from the supernatant of the culture.
Construction of a secondary phagemid library.
Screening of phage or phagemid libraries can be in liquid phase or in solid phase.
This resulted in infection of bacteria by more than one phagemid.
Described are both phage and phagemid vectors for surface display.
See also
The repertoire was treated as follows in order to rescue phagemid particles.
The term phagemid may also be used to connote these structures.
Functionality of pIII phagemid display.
Phagemid vectors are often employed for constructing the phage library.
Cosmid containing a concatamer of phagemid.
Phagemid library in vitro.
Kanamycin would allow selection of clones containing the phagemid.
Sequencing analysis identified two phagemid clones sharing a sequence overlap.
The insert in the phages was excised and cloned as a phagemid.
Various phagemid cloning systems to produce combinatorial libraries have been described by others.
A library used for panning may comprise a phagemid vector.
Bluescript phagemid inserts were excised from positive clones for further analysis.
These recombinant vectors can equally be cosmid or phagemid derivatives.
This phagemid vector has unique EcoRI and HindIII cloning sites.
Were used as the input phagemid.
On repackaging the same phagemid are obtained as were present before cleavage and ligation.
The filamentous phage may comprise a phage genome or a phagemid.
The phage is then isolated and the phagemid is excised with a helper phage.
Any colonies that grow contain the pure phagemid.
Only cells containing the phagemid grow on agar plates under kanamycin selection.
These fragments were cloned into a house made phagemid vector.
Phagemid clones were characterized by restriction enzyme mapping and partial nucleotide sequence analysis.
The resulting phage population is a mixture of phage abd rescued phagemid.
This constitutes the final antibody phagemid library which was used for selections.
Examples of such techniques include alanine scanning mutagenesis and phagemid display.
The cycles of phagemid selection are repeated until the desired affinity properties are achieved.
The package is then used to direct the phagemid to the target cell.
The nucleic acid may be comprised within a phage genome or within a phagemid.
After incubation the unbound phagemids were washed off and bound phagemid eluted.
In all embodiments of the invention filamentous bacteriophage particles containing a phagemid genome are employed.
Most preferred is a phage or phagemid vector comprising the signal sequence DsbA or a homolog thereof.
Mass excisions can be performed on these libraries to generate libraries in the pBluescript phagemid.
Phagemid particles were recovered from the repertoire as described earlier for the initial library.
Monovalent display can be accomplished with the use of phagemid and helper phage.
A phagemid is defined as a plasmid containing the packaging and replication origin of the filamentous bacteriophage.
