Examples of 'phagemid vector' in a sentence
Meaning of "phagemid vector"
phagemid vector: A type of vector used in molecular biology for cloning DNA sequences. It combines features of both bacteriophage M13 vectors and plasmid vectors. Phagemid vectors are commonly used for applications such as DNA sequencing and protein expression
How to use "phagemid vector" in a sentence
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phagemid vector
In preferred embodiments the display vector is a phagemid vector.
This phagemid vector has unique EcoRI and HindIII cloning sites.
A library used for panning may comprise a phagemid vector.
Most preferred is a phage or phagemid vector comprising the signal sequence DsbA or a homolog thereof.
These fragments were cloned into a house made phagemid vector.
Preparation of antibody phagemid vector for monovalent display of Fab fragments.
The Fab encoding sequence can then be subcloned into a phagemid vector.
A phagemid vector comprising Seq.
Resultant colonies contained the double stranded phagemid vector with the cloned cDNA insert.
If a phagemid vector is used, then this step requires superinfection with helper phage.
In preferred embodiments the expressible DNA sequence is in the form of a phagemid vector.
Construction of the phagemid vector and the initial humanized anti-VEGF.
In some embodiments, the vector is a phagemid vector.
A phagemid vector as in claim 1 wherein the nucleotide sequence encoding a product encodes a synthetic pIII.
Optionally, the expression vector is a phagemid vector.
See also
Phagemid vector pCES1 for display of antibody Fab fragments.
In some examples, the expression vector is a phagemid vector.
Clone as Sfil-NotI fragment into pCANTAB5myc phagemid vector to produce bispecific diabody repertoire.
Alternatively, the foreign protein or polypeptide may be expressed using a phagemid vector i . e.
A phagemid vector comprising,.
Construction of protease mutant phage libraries using the pMal-C2 phagemid vector.
An exemplary and preferred phagemid vector is the plasmid pC3AP313 described in the Examples.
In some embodiments the vectors are expression vectors, display vectors, such as a phagemid vector.
An example of a vector is the pHEN1 phagemid vector Hoogenboom et al.
In some embodiments, cDNA is cloned into a phage display vector, such as a phagemid vector.
ID No. 18 . A phagemid vector comprising a sequence selected from the group consisting of Seq.
This was derived from the spleen lymphocytes from 20 healthy donors and cloned into a phagemid vector.
For example, the phagemid vector is pNDS1.
Screening of scFv Supernatant from E. coli transformed with the recombinant phagemid vector was collected.
The phagemid vector pKN1 ( figure 9 ) was constructed in several steps as follows.
The Fab encoding sequence was subcloned into the phagemid vector phGHamg3.
A protease cleavable phagemid vector is derived from fd-K108 much as above except using pCANTAB 3 ( Pharmacia ).
This fragment was cloned into the pSELECT1 phagemid vector from Promega.
The library according to claim 1 or 2, wherein said DNA sequences are in a phagemid vector.
Figure 6 gives a schematic representation of the phagemid vector pUR4676 which was assembled from,.
A 1 kb PCR product was isolated and amplified by insertion into a Bluescript ™ ( Stratagene ) phagemid vector.
The amplified promoter was then blunt-end ligated into a phagemid vector ( pBluescript ® ; Stratagene, La Jolla, CA ).
For example, the host cell is E. coli . Optionally, the expression vector is a phagemid vector.
The Fab encoding sequence was subcloned into the phagemid vector phGHamg3 Bass et al.
The pSymvc20 vector can accept any Not I / Eco RI fragment from the phagemid vector.
These fragments were cloned into phagemid vector pAX50.
The pHAL14 vector is derived from the pHAL1 phagemid vector.
This PCR product was blunt-endedly subcloned into the phagemid vector pBluescript SK -.
These fragments were cloned into phagemid vector pASF20.
Figure 2B is a schematic representation of cloning phagemid vector pBK-CMV.
Coli strain XL1-Blue ( Stratagene ) is then transformed with the above phagemid vector containing Fd-cpIII.
Cloning and phage display, The library will then be cloned into the phagemid vector pCANTABhis6 [ 27 ].
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Examples of using Phagemid
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Phagemid preparations are prepared as recommended by the manufacturer
This constitutes the primary phagemid library
The phagemid was not affected by this treatment
