Examples of 'plasmid preparations' in a sentence
Meaning of "plasmid preparations"
Plasmid preparations: This phrase refers to the process of isolating and purifying plasmid DNA for use in genetic engineering experiments or research
How to use "plasmid preparations" in a sentence
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plasmid preparations
All plasmid preparations used were cesium banded.
The alkaline method was used for small scale plasmid preparations.
Crude plasmid preparations are made of the same set.
No bacterial genomic DNA was present in the plasmid preparations.
Two independent plasmid preparations were obtained for each construct.
No contamination with bacterial genomic DNA or RNA was present in the plasmid preparations.
Plasmid preparations and transformations.
Glycerol stocks and plasmid preparations were made from each plate.
Plasmid preparations from these colonies were analyzed by restriction enzyme digestion and gel electrophoresis.
Clycerol stocks and plasmid preparations were made from each plate.
Plasmid preparations were performed using Qiagen kits as per manufacturers instructions.
Candidate clones are then isolated and plasmid preparations are performed to obtain scFv sequence information.
Mixed plasmid preparations were made from six libraries using conventional techniques see Methods Enzymol.
Template for PCR reactions were plasmid preparations containing the target genes.
These plasmid preparations were used for automated DNA sequencing.
See also
The alkaline method ( Maniatis et al ., cited above ) is used for small scale plasmid preparations.
Large scale plasmid preparations were carried out for all of the mutant constructions described.
The alkaline method ( Maniatis et al ., cited above ) is used for small-scale plasmid preparations.
Small scale plasmid preparations from Lactococcus were done essentially according to Israelsen et al.
Coli INVaF ' ( Invitrogen, Carlsbad, CA ) was used for plasmid preparations.
Plasmid preparations of ZFN expression vectors constructed as described in FIG.
Two out of 16 plasmid preparations had lost the wild type Sau3A site.
Plasmid preparations were made and transformed into TG1 cells.
Transformation and plasmid preparations were according to that described by Maniatis, T., supra.
Plasmid preparations from these colonies were analyzed by restriction enzyme digestion ( Kpn I ) then gel electrophoresis.
First, the plasmid preparations of the individually isolated antigen-binding Fab clones are pooled.
Plasmid preparations from these colonies were analyzed by restriction enzyme digestion ( Kpn I ) and gel electrophoresis.
Small scale plasmid preparations were done on the E . coli transformants.
All plasmid preparations were banded at least once in CsCl / ethidium bromide gradients.
Large scale plasmid preparations were achieved with the Plasmid Maxi Kit ( Qiagen ).
All plasmid preparations were checked by a combination of restriction analysis and / or sequence analysis.
Therefore two DNA plasmid preparations of pUC12-gB685 were digested respectively by HindIII and XmnI.
The plasmid preparations were eluted with EB buffer and collected in 96-well plates.
Preparing plasmid preparations and extracts of host cells transfected with the products of ( a ) ;.
Plasmid preparations -- agarose gel electrophoresis of plasmid preparations distinguishes B. t.
Plasmid preparations were checked for the absence of lipopolysaccharide contamination ( Limulus assay, Sigma Chemical Co. ).
The purified plasmid preparations from these cultures constitute the pIJ922 - S . avermitilis genomic library.
Plasmid preparations -- agarose gel electrophoresis of plasmid preparations distinguishes PS81RR1 from B. t.
Plasmid preparations were performed using plasmid purification kits ( QIAGEN, Crawley, West Sussex, UK ).
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Examples of using Preparations
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An increasing number of preparations are being conducted on a regional basis
Preparations for these discussions are well advanced
Being in charge of the preparations for intergovernmental meetings
Examples of using Plasmid
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Said plasmid contains a tetracycline resistance gene
The construction of this plasmid is described below
Deposit of plasmid containing carcinogenic substance adducts
