Examples of 'primary antibodies' in a sentence
Meaning of "primary antibodies"
In the field of biology or immunology, primary antibodies are antibodies that directly bind to a specific target, such as an antigen or protein of interest. They are often used in various laboratory techniques, such as immunohistochemistry or Western blotting, to detect or study specific molecules
How to use "primary antibodies" in a sentence
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primary antibodies
Negative controls were performed omitting primary antibodies.
The following primary antibodies were used.
Negative controls were performed without primary antibodies.
Primary antibodies used to recognize the initial markers.
Direct detection utilizes labelled primary antibodies.
Primary antibodies were incubated overnight at room temperature on a shaker.
Control sections were stained without primary antibodies.
Summary of the primary antibodies used in this study.
Negative control was prepared in the absence of primary antibodies.
List of primary antibodies used.
Tissue sections were incubated overnight with primary antibodies.
Cells untreated with primary antibodies were used as negative controls.
For negative controls we omitted the primary antibodies.
It is possible to use primary antibodies directly conjugated to fluorescent tags.
Negative controls were performed by omitting the primary antibodies.
See also
Cells untreated with primary antibodies are used as negative controls.
Antibodies to the binding proteins are herein referred to as primary antibodies.
In control experiments primary antibodies are omitted.
Primary antibodies used were.
Were incubated with primary antibodies.
Omission of primary antibodies was used as a negative control and showed no staining.
Appropriate isotype controls were used at the same dilutions as primary antibodies.
Overnight in primary antibodies.
Immunostaining is performed accordingly to conventional method using multiple primary antibodies.
Cells were incubated with appropriate primary antibodies for one at room temperature.
The primary antibodies and secondary antibody conjugates were applied as outlined above.
Wash briefly and incubate with the primary antibodies overnight.
Both primary antibodies can be used at the same time on the same sample.
The chamber allows the incubation of a blot with different primary antibodies.
Enzymes may be conjugated to primary antibodies by any one of several common methods.
Normal donkey serum was used when staining with goat primary antibodies.
This saves the cost of modifying the primary antibodies to directly carry a fluorophore.
All primary antibodies are therefore bound to the solid phase vÃa the second antibody.
Negative controls consisted of cells stained with the primary antibodies omitted.
These primary antibodies may be directly labeled or may be detected with suitable secondary antibodies.
Protein expression was subsequently detected with primary antibodies against the different antigens.
The deposition of specific chromogens of different colors represented the localization of the primary antibodies.
In another embodiment each one of multiple primary antibodies contains a distinct and detectable label.
Primary antibodies are used then appropriate species and chain specific control antibodies should be used.
Background fluorescence was estimated by substituting the specific primary antibodies with specific isotype controls.
IgM antibodies are primary antibodies released into the blood early in the immune response.
The secondary antibodies are not antibodies against either the nonimmunogenic materials nor the primary antibodies.
Binding of the primary antibodies was detected with an antimouse secondary antibody and DAB chromagen.
The washed NC membrane was reacted with primary antibodies.
The list of primary antibodies and their working dilutions are shown in Table LA.
Second antibodies include species specific Rhodamine conjugated antibodies corresponding to the primary antibodies.
The list of primary antibodies and their working dilutions are shown in Table IB.
Second antibodies included species specific Rhodamine conjugated antibodies corresponding to the primary antibodies.
The used primary antibodies were available from Cell Signaling Technology and others.
Method specificity was verified by substituting primary antibodies with PB or NGS.
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