Examples of 'primary antibody' in a sentence
Meaning of "primary antibody"
primary antibody - In biology and research, a primary antibody is an antibody that specifically binds to a target antigen or protein of interest. It is used to detect, measure, or purify the target antigen in various laboratory techniques like Western blotting, ELISA, or immunohistochemistry
How to use "primary antibody" in a sentence
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primary antibody
Most primary antibody classes are of IgG class.
Preferably leukocytes are bound by the primary antibody.
Incubate with primary antibody solution overnight.
Control stainings were performed by omitting primary antibody.
Dilutions of each primary antibody were as described below.
Controls included omitting the primary antibody.
Controls without primary antibody were all negative.
Run a secondary control without primary antibody.
The primary antibody is conjugated to alkaline phosphatase.
This method uses a labeled primary antibody.
The primary antibody was omitted for negative controls.
As negative control no primary antibody is used.
Primary antibody is added which binds to the antigen.
Controls without primary antibody were performed.
Primary antibody was then added to the fixed cells.
See also
Calculate volume of primary antibody needed.
The primary antibody is the test sample or admixture.
In certain embodiments the primary antibody may be polymerized.
The primary antibody has a means of detection conjugated to it.
Cells are incubated with a primary antibody recognizing an antigen.
A fluorophore can be directly conjugated to the primary antibody.
Omission of the primary antibody served as a negative control.
The negative control was incubated without primary antibody.
Omission of the primary antibody resulted in absence of labeling.
Membranes were incubated with the primary antibody overnight.
The primary antibody is then added.
It is the most common of the primary antibody deficiencies.
Optimum primary antibody was determined from saturation kinetics.
Host species of the primary antibody.
Omission of the primary antibody was used as a negative control.
Membrane was then incubated with a primary antibody at the.
Omission of the primary antibody was the negative control.
Control samples were incubated in the absence of primary antibody.
One of these is the primary antibody described above.
Negative controls were stained by omitting the primary antibody.
Not enough primary antibody is bound to the protein of interest.
The supernatant of monoclonal cell was used as primary antibody.
The primary antibody may be any mouse IgG isotype.
Triplicate wells were set up for each primary antibody.
Immunoreactivity of the primary antibody may be reduced as a result of labeling.
The supernatant of monoclonal cells was used as primary antibody.
This primary antibody was conjugated to alkaline phosphatase as described above.
This was then used as a primary antibody.
The primary antibody and the secondary antibody were as follows.
Negative controls were obtained by omitting the primary antibody.
Cells stained without the primary antibody were used as controls.
The primary antibody is preferably an antibody which binds to presepsin.
Tissue stained in the absence of primary antibody were negative controls.
It is the primary antibody produced in the secondary immune response.
Negative control reactions were obtained by omitting the primary antibody.
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