Examples of 'protein a chromatography' in a sentence
Meaning of "protein a chromatography"
protein a chromatography - a laboratory technique used to separate and purify proteins based on their properties
How to use "protein a chromatography" in a sentence
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protein a chromatography
The recombinant chimeric antibody was purified from the conditioned medium using standard protein A chromatography.
The solid phase for the protein A chromatography is equilibrated with a suitable buffer.
Antibody was purified from culture supernatants by protein A chromatography.
Protein A chromatography from murine ascitic fluid.
Antibody was purified from tissue culture supernatant by protein A chromatography.
Preferably the Protein A chromatography procedure described herein is used.
Antibodies were purified from cell culture supernatant by protein A chromatography.
To remove the oxidizing impurity, protein A chromatography with salt washes of varying strength was assessed.
The medium used in a method described herein is a Protein A chromatography column.
CTLA4Ig was purified by protein A chromatography from serum-free conditioned supernatants ( Figure 2 ).
Fc fusión constructs were captured from concentrated culture supernatants by Protein A chromatography.
Antibody titers were measured by Protein A chromatography on the final day.
The Fab fragment was separated from intact antibody and Fc fragments by Protein A chromatography.
Contacting the eluate recovered from a Protein A chromatography column with activated carbon ;.
Secreted antibody was purified from the cell culture supernatants by Protein A chromatography.
See also
The medium can be, without limitation, a Protein A chromatography column or a Protein G chromatography column.
The mAb in the spiked lysate was then captured with Protein A chromatography.
However, there are numerous drawbacks to using Protein A chromatography for protein purification.
The chromatography step may be cation exchange chromatography, mixed mode chromatography, or Protein A chromatography.
Another buffer that is typically employed during Protein A chromatography is a loading buffer.
Table 6 illustrates a comparison of the performances of oilbodies with protein A chromatography.
The Fc proteins were purified using standard protein A chromatography ( 5ml column, Pierce ).
The sterile filtered solution is the loading material for the Protein A chromatography.
Purification of alemtuzumab using protein A chromatography.
Preferably, the antibody purification comprises a step of affinity chromatography, more preferably Protein A chromatography.
Such an affinity purification can be over a Protein A chromatography medium.
The mAb was purified to > 95 % homogeneity by precipitation with ammonium sulfate followed by protein A chromatography.
In a preferred embodiment, the medium is a Protein A chromatography column.
The ocrelizumab antibody was purified immediately ( t = 0 hr ) from the initial mixture using protein A chromatography.
In a specific embodiment the purifying is by Protein A chromatography.
Recombinant RSPO1-Fc was then purified from the conditioned insect medium using protein A chromatography.
Both Fc fusión proteins were purified by protein A chromatography.
The methods for purifying TACI-Fc that are available in the art involve a Protein A chromatography.
The latter are generally not separated using Protein A chromatography.
Wild type and variants of alemtuzumab were expressed in 293T cells and purified with protein A chromatography.
Purification of UTI-Fc fusions was done by Protein A chromatography.
IgGs were expressed in 100 mL transfected CHO cell cultures and purified by protein A chromatography.
CV1q was purified from cell supernatant by protein A chromatography.
The anti-HeliCar motif amino acid sequence antibody ( 0019 ) was purified by standard procedures using protein A chromatography.
Specifically, the binding affinity of humanized 21M18 antibodies purified by protein A chromatography was determined using Biacore.
All mutants were purified from CHO culture supernatants by protein A chromatography.
The culture supernatant is collected and purified by Protein A chromatography.
Field of the invention The present invention provides methods for cleaning a Protein A chromatography column.
In some embodiments, the polypeptide is in an eluent from a Protein A chromatography.
Most preferably, enrichment in IgG antibodies is performed by protein A chromatography.
However, there are numerous drawbacks to the use of Protein A chromatography.
All proteins were purified by a two-step procedure, first using protein A chromatography.
Clarified culture supernatant was purified using one-step Protein A chromatography.
About 3 days post-transfection, the supernatant was purified by Protein A chromatography.
Antibodies GenO.95 was purified from cell culture supernatant by Protein A chromatography.
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