Examples of 'protein a sepharose' in a sentence
Meaning of "protein a sepharose"
protein a sepharose: Refers to a laboratory technique or material used in protein purification processes
How to use "protein a sepharose" in a sentence
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protein a sepharose
Pure mAbs are prepared by protein A sepharose purification of IgG from hybridoma culture supernatants.
The recombinant fusión proteins were purified to homogeneity on protein A sepharose.
The pure protein was isolated by Protein A sepharose fast flow affinity chromatography.
A protein A sepharose column was used to capture the Fc portion of the fusión proteins.
The protein was expressed in baculovirus and purified with protein A sepharose column.
The fusión proteins were purified vía protein A Sepharose chromatography according to the manufacturers instructions.
Expression culture supernatants were filtered and antibodies were purified using protein A sepharose.
The protein A sepharose pellet can be discarded.
The antibody is purified from cell culture supernatant on a protein A sepharose column.
One ml of Protein A Sepharose was added overnight to the reaction mixture with gentle shaking.
The antibodies were then isolated from the ascites fluids by using protein A Sepharose.
The clarified supernatant was passed over a Protein A Sepharose column which had been equilibrated with PBS.
The trace amount of radiolabelled domains produced is mixed with antibody samples and protein A Sepharose.
After dialysis the mixture was purified on a protein A Sepharose column equilibrated with PBS.
Será were collected and the anti-MAdCAM antibodies isolated by affinity chromatography on protein A sepharose.
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Serum obtained was further purified on protein A sepharose ( Repligen ) to obtain the IgG fraction.
Positive clones are further expanded, sub-cloned and supernatants are purified using a protein A sepharose column.
Purified antibodies were obtained by Affigel protein A Sepharose chromatography ( BioRad ) according to the maufacturer 's manual.
The proteins were expressed in CHO cells and purified using a protein A sepharose column.
Protein A Sepharose Beads / antibody mixtures have been incubated 1 hour at room temperature under constant rotation.
G immunoglobulins are purified on a protein A Sepharose column.
Pure mAbs were prepared by Protein A Sepharose purification ( Pharmacia ) of IgG from culture supernatants.
It was purified from ascites fluid by using protein A Sepharose.
Secreted IgG was purified using protein A sepharose ( Pharmacia ) and buffer exchanged into PBS.
The culture supernatant, containing the antibody, was purified by protein A sepharose chromatography.
After the incubation, the protein A sepharose beads were removed and added to STF293 cells.
Antibodies were purified using protein A sepharose.
Protein A sepharose beads were added, and allowed to incubate at 4°C for 1 h.
The fusión protein can be purified on protein A Sepharose columns.
Protein A sepharose ( Pharmacia ) was added and the incubation was continued for 30 ' with rotation.
The resulting fusión protein was expressed and purified by Protein A Sepharose chromatography.
The A41L fusión proteins were purified by Protein A sepharose affinity chromatography according to standard procedures.
Briefly, culture supernatants were loaded onto a 1 ml protein A sepharose column.
Add 50µL of protein A sepharose beads.
Next, a sample of the será can be incubated with protein A Sepharose.
In brief, antibodies are applied to a Protein A Sepharose column ( GE healthcare ) and washed with PBS.
The elk, Fc fusión protein was purified by affinity chromatography, using a protein A sepharose column.
Secreted antibodies were purified using protein A Sepharose ( Pharmacia ), then dialyzed with PBS.
Cells were removed by centrifugation, lysed in Crumpton lysis buffer and precleaned with protein A Sepharose.
Immune complexes were adsorbed with protein A sepharose at 4°C overnight.
TRU-016 was purified from cell culture supernatants initially by affinity chromatography using Protein A Sepharose.
Immuno-complexes were then captured with 20 µl Protein A Sepharose slurry by 1 h incubation under rotation.
After digestion with LysC, the Fc containing proteins were removed with Protein A Sepharose.
Positive antibodies were purified from hybridoma cultures by protein A Sepharose chromatography ( Amersham ).
The receptor / Fc constructs were precipitated by incubation with Protein A sepharose beads.
DAbs were purified from 50 ml culture supernatants using protein A Sepharose in a batch-wise manner.
Protein was immunoprecipitated from lysates of transfected cells using IM140 coupled to Protein A Sepharose beads.
The dialysate is then run through an affinity column, such as a protein A Sepharose bead column.
We further purified the rsELAM1 by chromatography with Protein A sepharose in PBS.
Lysates were immunoprecipitated with the designated antibody immobilized on Protein A Sepharose ( BioRad ).
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