Examples of 'protein a-sepharose' in a sentence
Meaning of "protein a-sepharose"
protein a-sepharose - Refers to a type of chromatography resin used for purification of proteins. It is designed to specifically bind proteins containing the Staphylococcal protein A domain
How to use "protein a-sepharose" in a sentence
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protein a-sepharose
The different monoclonal antibodies were isolated using protein A-Sepharose.
After intensive washing, protein A-Sepharose was pelleted and transferred to counting tubes.
The antibodies were purified by chromatography on protein A-sepharose.
Immunoprecipitation with protein A-sepharose was followed by frequent washing.
Antibodies in the conditioned media were purified on Protein A-Sepharose.
Complexes were collected with protein A-Sepharose and washed with DIB.
Antibodies in the conditioned media were purified with Protein A-Sepharose.
The immunocomplexes are collected on protein A-sepharose beads and then separated by polyacrylamide gel electrophoresis.
Clarified conditioned media were loaded onto protein A-sepharose column.
Protein A-Sepharose was added and incubations continued for 2 hours.
The IgG fraction was prepared by protein A-sepharose chromatography.
Fc fragments and undigested immunoglobulins are removed by affinity chromatography on Protein A-Sepharose.
IgGs were purified by affinity chromatography on protein A-sepharose columns by standard methodologies.
JNK was isolated by immunoaffinity chromatography using the M2 monoclonal antibody bound to protein A-Sepharose.
Monoclonal antibodies were purified on a protein A-Sepharose column ( electrical engineering biosciences ).
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Crude supernatants were harvested, sterile filtered and purified by protein A-sepharose.
The protein A-Sepharose was then washed 20 times to remove non specifically bound iodinated protein.
Antibody-bound and free adipsin are separated by absorption to protein A-sepharose or by charcoal absorption.
Protein A-sepharose was added to the mixture and incubated for an additional 2 h.
The resulting chimeric A2 antibody is purified from tissue culture supernatant by protein A-Sepharose chromatography.
Protein A-Sepharose was then added and the samples were further incubated for 3 hr at 4°C.
The antibody Fc portions and undigested antibodies were removed by passage over a protein A-Sepharose 4B column.
The sample is allowed to bind to the protein A-sepharose for approximately 30 minutes.
Undigested Ig and Fc fragments were removed by chromatography on protein A-Sepharose.
The pelleted Protein A-Sepharose was resuspended and tubes were incubated at 4°C for 1 hr with agitation.
The monoclonal antibodies in the ascitic fluid were purified through a Pharmacia protein A-sepharose affinity column.
It was then applied to a Protein A-Sepharose CL4B column ( commercially available from Pharmacia ).
The immunocomplexes were isolated by adsorption to Protein A-sepharose.
Immunoprecipitations were performed using protein A-sepharose ( Pharmacia ) coupled to specific anti-bodies.
Chromatography column is prepared using 12 ml of protein A-sepharose.
The protein A-Sepharose complexes were washed once with PBS and then subjected to SDS-8 % PAGE.
The complexes were precipitated with Protein A-sepharose beads.
Protein A-Sepharose ( 300 µL ) was added to each tube and the resin pelleted by centrifugation.
Antibodies were purified by passage over a column of staphylococcal protein A-Sepharose CL-4B.
Ig1 T, rabbit immunoglobulins purified by protein A-sepharose from blood withdrawn after immunization with VEGF.
At that time, the lysates were transferred to tubes containing 50 µl of packed Protein A-sepharose.
The resultant precipitate was subjected to treatment with Protein A-Sepharose CL-4B ( Pharmacia ) for antibody purification.
Undigested IgG molecules, and Fc-fragments were removed by affinity chromatography with protein A-sepharose.
Immunocomplexes were absorbed to 150 µ l protein A-Sepharose beads ( PAS, Pharmacia ) and isolated by centrifugation.
Supernatants were collected 3-4 days post transfection and the antibodies were purified via protein A-sepharose chromatography.
IgG was isolated on a protein A-Sepharose column ( Pharmacia ).
Antibodies were purified from the single stable cell line supernatants using protein A-sepharose ( Pharmacia ).
Rse-IgG was purified on a protein A-Sepharose column ( Pharmacia ).
The bound complex was immunoprecipitated with anti-alkaline phosphatase polyclonal antibodies ( Zymed ) and protein A-Sepharose ( Repligen ).
Mer-Fc was purified on a protein A-sepharose column ( Pharmacia ).
Harvested cell culture fluid containing rhuMAb VEGF was purified using protein A-Sepharose CL-4B.
NAP-5 and Protein A-Sepharose columns were purchased from Pharmacia ( Sweden ).
The fusión protein was then purified using Protein A-Sepharose ( Sigma ).
Peptide fusion-antibody complexes were precipitated with Protein A-sepharose.
Said two antibodies were purified on a protein A-Sepharose 4B column ( Pharmacia ).
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