Examples of 'q-sepharose column' in a sentence
Meaning of "q-sepharose column"
q-sepharose column: A type of chromatography column used for protein purification and separation based on ion exchange. ~ context: Commonly used in biochemistry and molecular biology research for separating proteins based on their net charge
How to use "q-sepharose column" in a sentence
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q-sepharose column
The Q-Sepharose column chromatography was performed as follows.
The resulting mixture is then applied on a Q-Sepharose column.
The chromatogram from the Q-Sepharose column showed two major protein peaks.
The diluted medium is loaded onto a Q-sepharose column.
The protein peaks from the Q-sepharose column were pooled and loaded onto a Ni-NTA agarose column.
The diluted medium was loaded onto a Q-sepharose column.
The protein peaks from the Q-sepharose column are pooled and loaded onto a Ni-NTA agarose column.
Further purification was performed on a Q-sepharose column.
A single Q-sepharose column is optionally used for purification of OXDC.
This last stage is carried out by preferably using a Q-Sepharose column or an equivalent column.
A Q-Sepharose column was prepared in a volume of 1 ml per 5 ml diluted detergent phase.
To separate impurities the dialysate was applied to a Q-sepharose column.
The Q-Sepharose column resolved AUXI and AUXII collagenases as expected ( figure 72 ).
The dialyzed protein was then loaded onto a Q-sepharose column and eluted using a salt gradient.
The 69 kDa protein was applied to a hydroxylapatite column connected in tandem with a Q-Sepharose column.
See also
The supernatant was taken and run through a Q-sepharose column ( Pharmacia ).
Fractions containing LPP ( s ) - SHe were pooled, desalted and further purified on a Q-sepharose column.
Endotoxin was mainly removed during the Q-sepharose column step, see Table 1.
A sample of about 250 ml is obtained which is loaded on the Q-Sepharose column.
The resulting soluble fraction was applied to a 2.6 x 10 cm Q-Sepharose column equilibrated with the buffer A.
A sample of about 360 ml is obtained which is chromatographed on the Q-Sepharose column in 3 portions.
Using this assay, CNTF washes through the Q-Sepharose column in Buffer B.
The resulting soluble fraction was charged to a 2.6 x 10 cm Q-Sepharose column equilibrated with the buffer A.
The dialyzate was neutralized with 1M NaOH and chromatographed on a Q-Sepharose column ( Pharmacia, Piscataway, NJ ).
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