Examples of 'sepharose column' in a sentence
Meaning of "sepharose column"
sepharose column: In the context of biochemistry or molecular biology, a Sepharose column is a type of chromatography column used for protein purification. Sepharose is a gel matrix composed of cross-linked agarose beads, which helps separate biomolecules based on size, charge, and other properties
How to use "sepharose column" in a sentence
Basic
Advanced
sepharose column
Enzyme was purified by means of a glutathione sepharose column.
Further fractionation was carried out on a sepharose column which separates according to molecular size.
The elastase digestion mixture was applied onto a lysine Sepharose column.
Protein was purified on a Q Sepharose column for polyclonal antibody production in rabbits.
The mixture was applied onto the phenyl sepharose column.
The eluate of the DEAE Sepharose column is adjusted to the specified conductivity.
The supernatant was desalted and applied to a Blue Sepharose column.
A protein A sepharose column was used to capture the Fc portion of the fusión proteins.
This step is carried out preferably using a Q Sepharose column or an equivalent column.
The monoclonal antibodies were purified by separating the IgG fraction with a Protein A bound Sepharose column.
Solubilised pertactin was loaded onto a chelating Sepharose column charged with zinc and equilibrated with buffer A.
Enzymatically active fractions were pooled and loaded onto a Phenyl Sepharose column.
The clarified supernatant was passed over a Protein A Sepharose column which had been equilibrated with PBS.
The protein was expressed in baculovirus and purified with protein A sepharose column.
A vpr - CnBr activated Sepharose column was also used.
See also
The antibody is purified from cell culture supernatant on a protein A sepharose column.
A protA sepharose column was used to remove remaining intact antibodies and / or Fc fragments.
The thrombin was purified from this preparation by binding to Blue Sepharose column.
Affinity chromatography using a Blue Sepharose column ( Pharmacia ) was then used as an additional purification step.
The proteins were expressed in CHO cells and purified using a protein A sepharose column.
The Ni-IDA Sepharose column was washed consecutively with,.
Coli western blot also demonstrated elimination of most HCP already after the Q sepharose column.
Human PRP ( h-PRP ) was applied to a Sepharose column for the purification of platelet fractions.
G immunoglobulins are purified on a protein A Sepharose column.
The corresponding capacity for the HSA Sepharose column was determined to 0.5 mg per ml resin.
Purification was performed by affinity chromatography on a Lentil Lectin Sepharose column.
The eluent fractions from the DEAE Sepharose column were evaporated to < 20 mL.
The crude UNG preparations are next passed through a DEAE Sepharose column.
For panel B sepharose column purified recBIIA3-immune IgG was used.
After dialysis the mixture was purified on a protein A Sepharose column equilibrated with PBS.
Pool containing lipolytic enzyme activity was then appllied on The High performance Q sepharose column.
Further purification of OspA-B31 on a SP Sepharose column resulted in a single band by SDS-PAGE.
The five best clones were chosen for purification on an antigen-coated sepharose column.
The product is passed through an S Sepharose column ( H ⁺ form ) equilibrated in methanol.
The supernatant was filtered, and then it was slowly passed through the above-described sepharose column.
In brief, antibodies are applied to a Protein A Sepharose column ( GE healthcare ) and washed with PBS.
If the chimeric protein is soluble, it can be purified on a Sepharose column.
The protein solution was applied to a hIgG sepharose column ( 50 mg hlgG per ml sepharose 4B ).
Ascites were then collected and purified over a protein-A Sepharose column.
In addition, vpr protein was collected off a DEAE sepharose column using a salt gradient.
The bispecific molecules were further purified by hydrophobic interaction chromatography ( HIC ) on a Phenyl Sepharose column.
Following dialysis, proteins were applied to a Q sepharose column ( Pharmacia Biotech ; Piscataway, NJ ).
Specifically, the filtered culture medium was loaded on the recombinant protein-A Sepharose column.
The monoclonal antibodies were purified using the Protein A-bound Sepharose column to separate IgG fractions.
If desired, the antibodies then can be affinity purified using a Protein-A Sepharose column.
Fraction IIb was loaded onto the phenyl sepharose column at 78 ml / hr.
Q-pool is the pooled active fractions of bovine spinal cord extract separated on a Q sepharose column.
The latter solution was immediately loaded onto the CM sepharose column at 31.4 L / hour.
Positive clones are further expanded, sub-cloned and supernatants are purified using a protein A sepharose column.
The labelled protein was then purified on a G50 sepharose column ( Pharmacia Inc. ).
You'll also be interested in:
Examples of using Sepharose
Show more
In one embodiment the solid support is sepharose
Sepharose has been found to be suitable for this purpose
Enzyme was purified by means of a glutathione sepharose column
Examples of using Column
Show more
This column also captures those situations
This marks my final column in this magazine
A column matrix is a matrix with a single column
