Examples of 'recombinases' in a sentence
Meaning of "recombinases"
Recombinase is an enzyme that is capable of catalyzing the process of genetic recombination, playing a key role in the rearrangement of genetic material
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- plural of recombinase
How to use "recombinases" in a sentence
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recombinases
Recombinases are genetic recombination enzymes.
Mechanistic studies of the cre and flp recombinases.
Recombinases are found in all three kingdoms of life.
Kanamycin selection scheme for detecting improved recombinases.
Preferably the site specific recombinases are XerC and XerD.
Such recombinases are known.
Linkage is then performed by adding the recombinases facilitating recombination.
Each of these recombinases recognize specific target integration sites.
Site specific recombinases.
Use of tailored recombinases for the treatment of retroviral infections.
Proteins responsible for homologous recombination are collectively called recombinases.
Single clones of recombinases were selected and were subjected to further analyses.
In another embodiment the endogenous site specific recombinases may be transposases.
In addition these recombinases do not require auxiliary factors to function.
This testing is performed prior to preparing said recombinases as pharmaceutical composition.
See also
The efficiency of transformation is increased by the expression of site specific recombinases.
The mechanism and control of serine recombinases is much less well understood.
The circuits in the biological state machine are dependent on enzymes called recombinases.
Recombinases are key enzymes that catalyse the strand transfer step during recombination.
Said activity includes but is not limited to recombinases and integrases.
The lambda recombinases promote homologous recombination between nucleic acids in the cell.
Typically the group of plasmids represents a shuffled library of recombinases or mutagenized recombinases.
Altered recombinases may be generated from each of these recombinases by the methods described herein.
More preferably the XerC and XerD site specific recombinases are endogenous.
Recombinases may be used to excise the region of DNA.
In many cases the purification of such other recombinases has been described in the art.
The construct may also contain genes coding for one or more recombinases.
Mapping of mutations of evolved recombinases onto Cre secondary structure.
A representation of the directed evolution strategy used to evolve lox ltr specific recombinases is shown.
Conservative sequence specific DNA recombinases have been divided into two families.
The methods described herein allow the identification of coding sequences for altered recombinases.
In yeast and other eukaryotic organisms there are two recombinases required for repairing DSBs.
Zre recombinases recombine DNA at zox sites.
Such methods utilize the targeting constructs and altered recombinases of the present invention.
The best performing shuffled recombinases are retained and used in subsequent rounds of DNA shuffling.
Cre recombinase or FLP recombinase are preferred recombinases.
Most preferably the recombinases are Cre and FLP.
The FRT sites are recognition sites for the Flp recombinases.
Recombinases generally are integrases, resolvases or flippases.
An exemplary library comprises Cre and recombinases derived therefrom.
In one aspect, recombinases and integrases are used as regulatory domains.
The loxP and FRT sites are recognition sites for the Cre and Flp recombinases.
Several site-specific recombinases are known in the art.
In a preferred embodiment, sets of RTSs from different recombinases are used.
In one embodiment, recombinases and integrases are used as regulatory domains.
In this specific case, these are the recombinases XerC and XerD.
Such recombinases have been described in archaea, bacteria, eukaryotes and viruses.
Site-specific integration of transgenes directed by recombinases has previously been described.
Site-specific recombinases can be clustered into two major families.
In the present invention, such shuffling protocols have been used to generate altered recombinases.
