Examples of 'refolding buffer' in a sentence
Meaning of "refolding buffer"
refolding buffer: This phrase is commonly used in the context of molecular biology or biochemistry to describe a substance or solution that aids in the process of refolding proteins
How to use "refolding buffer" in a sentence
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refolding buffer
Inclusion bodies were solubilized and refolded by dilution in a refolding buffer.
Each tube containing refolding buffer components was brought to a final volume of 4 mL.
In other embodiments, the plasminogen is dialyzed against the refolding buffer.
Five-fold dilution into refolding buffer may also be used.
Alternatively, the polypeptide may be dialyzed against the refolding buffer.
If proteins contain disulfide bonds, the refolding buffer has to be supplemented with a redox system.
Alternatively, the neurotrophin may be dialyzed against the refolding buffer.
In one embodiment the refolding buffer comprises ethanolamine and SB-12.
Organic, water-miscible solvents can replace the detergent in the refolding buffer.
In this preferred embodiment the refolding buffer comprises SB-12 and ethanolamine.
Therefore, the solubilizate is preferably subjected to filtration prior to diluting with the refolding buffer.
Preferably the refolding buffer has pH11.
For heterodimers, the media uses CHAPS as the refolding buffer.
The pH of the refolding buffer was adjusted to 7.5.
Preferably, the solubilizate is added to the refolding buffer under stirring.
See also
In some embodiments, the refolding buffer comprises at least 0.01 mM of a calcium salt.
The respective inclusion bodies were dissolved in 8M urea solution and diluted by a refolding buffer.
A total of 15-20 column volumes of refolding buffer were applied in an overnight reaction.
Protease was stored as a stock preparation at 10 µM in refolding buffer.
Preferably the refolding buffer comprises ethanolamine and SB-12.
In one preferred embodiment of the present invention, the refolding buffer further contains arginine-HCl.
Alternatively, phospholipids can replace the detergent or the organic, water-miscible solvent in the refolding buffer.
ADI was eluted using refolding buffer containing 0.2 M NaCl.
The column was then further washed with 25 ml of refolding buffer.
Diafiltration is performed against 4 volumes of refolding buffer ( see above ).
In some embodiments, the final concentration in the refolding buffer is at least 0.01 mM.
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