Examples of 'replicon' in a sentence
Meaning of "replicon"
replicon: A unit of replication in genetics, typically referring to a segment of DNA that can replicate autonomously
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- A DNA molecule or a region of DNA that replicates as an individual unit. A replicon may be, for instance, a chromosome, a plasmid or a phage.
How to use "replicon" in a sentence
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replicon
Alphavirus vector or replicon systems have been described.
Additionally a replicating vector will contain a replicon.
These replicon constructs were prepared as described above.
Additionally a replicating vector might contain a replicon.
Said replicon is optimised according to the invention.
Compound dilution and application to replicon cell trays.
The replicon constructs can be bicistronic subgenomic replicons.
The organization of the replicon is indicated.
A replicon has to have an origin of replication.
Alphavirus vectors typically are constructed in a format known as a replicon.
A replicon may include operably linked expression control sequences.
Alphavirus and alphavirus replicon particle formulations and methods.
Mean value from multiple experiments of same laboratory replicon.
A runaway replicon is a temperature sensitive plasmid copy control mechanism.
Construction of improved replicon vectors.
See also
Alphavirus replicon particles matched to protein antigens as immunological adjuvants.
Chimeric alphavirus replicon particles.
The vector may or may not include a eukaryotic replicon.
An alphavirus replicon vector comprising.
Vero cells were electroporated with helper and replicon RNAs.
In an embodiment of the present invention replicon particle preparation potency is quantified.
The vector may or may not comprise a eukaryotic replicon.
Alphavirus replicon particles are administered as a booster some time after priming.
The present recombinant nucleic acids may be comprised on a replicon.
Plasmids possessing a temperature sensitive replicon are capable of being manipulated in this fashion.
The replicon may also be engineered to express alphavirus structural proteins.
Another strategy is to utilize a replicon vector to express the immunizing antigens.
Recombination sites will be completely eliminated from the replicon by splicing.
Wild type replicon cells in the absence of the compound were used as a control.
Each sequence of interest may preferably be expressed from one replicon.
Next the culture supernatants containing the replicon particles are harvested.
The suppressor gene may be located on the chromosome or on an extrachromosomal replicon.
Crude or clarified alphavirus replicon particles may be concentrated and purified by chromatographic techniques e.
The overlapping cDNA fragments were assembled into the contiguous replicon sequence.
Such a replicon would be comparable to viral vectors in conventional plant viral expression systems.
Selecting a transformed lactic acid bacterium in which the introduced replicon is capable of replicating.
Replication of this viral replicon will result in a high number of copies of the replicon.
The luciferase reporter assay was also used to confirm compound potency in the replicon.
A preparation of alphavirus replicon particles may be purified by a chromatographic purification process as described above.
This present invention describes a novel method by which to deliver replicon expression vectors.
The preparation of replicon particles being capable of expressing an antigen derived from a pathogenic agent.
These results can be explained only by the maintenance of the replicon inside the cell.
Thus a replicon useful with the disclosure may have two open reading frames.
The expression constructs of the present invention may or may not include a eukaryotic replicon.
In one preferred embodiment a replicon according to the invention is derived from a human immunodeficiency virus.
This approach provides a mechanism to exploit desirable properties from multiple alphaviruses in a single replicon particle.
Proteins necessary for replicon encapsidation include proteins that are part of the capsid structure.
Systemic infection of the plant may take place if the replicon encodes infectious viral particles.
Such replicon systems include one or more helper nucleic acid and one or more replicon.
There are also disclosed herein methods of producing viral replicon particles using packaging cell lines.
