Examples of 'restriction digestion' in a sentence
Meaning of "restriction digestion"
restriction digestion: A molecular biology technique used to cut DNA at specific sequences for further analysis or manipulation
How to use "restriction digestion" in a sentence
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restriction digestion
Recombinants are analysed by restriction digestion with HindIII.
The restriction digestion was evaluated by agarose gel eletrophoresis.
The fragment can be oriented by restriction digestion.
Restriction digestion with enzymeHindIII.
Recombinants are screened by starndard restriction digestion analysis.
Restriction digestion of the plasmids generated expected fragments on agarose gels.
This deletion was confirmed by restriction digestion of the plasmid.
Testing for homologous recombination leading to insertion of the transgene is done by restriction digestion.
Positive clones were confirmed by restriction digestion and sequencing.
Restriction digestion can be carried out using techniques and enzymes well known in the art.
All plasmids were confirmed by restriction digestion and sequencing.
Amplification occurs when the locus comprising the two primers remains intact following a restriction digestion.
Recombinants were characterised by restriction digestion with EcoRl and HindIII.
Several positive blue colonies were screened and identified by restriction digestion.
The fragments are generated by restriction digestion using the indicated enzymes.
See also
Recombinant plasmids are isolated and analyzed by restriction digestion.
Transformants were screened by restriction digestion to identify the desired recombinant plasmids.
Absence of the site can be confirmed by restriction digestion.
Positive clones were identified by restriction digestion and subsequently confirmed by sequence analysis.
The phagemids were then grown and analyzed by restriction digestion.
Positive clones were screened by restriction digestion for the presence of the inserted cassette.
Cross checking for the presence of insert by restriction digestion.
Restriction digestion procedures were performed by the methods of Sambrook et al.
The orientation was confirmed by restriction digestion.
Transductants were analysed by restriction digestion and Southern hybridisation before use as inoculum.
The resistant colonies were harvested and characterized by restriction digestion.
This modification was verified by restriction digestion and agarose gel electrophoresis.
The resulting colonies were harvested and characterized by restriction digestion.
Positive clones were identified by restriction digestion analysis with HindIII and EcoRI.
RFLPs are most easily detected by size fractionation methods following restriction digestion.
All sequences were verified via restriction digestion and sequencing.
The orientation of the CMV transcriptional control region was confirmed by restriction digestion.
One clone was selected for corn transformation by restriction digestion analysis for correct insert size.
Schematic representation of the GL and truncated forms generated by PCR or restriction digestion.
And extended to ends formed by restriction digestion Steigerwald et al.
Preferably the Entry Vector was dephosphorylated and gel purified after restriction digestion.
Appropriate assembly can be verified by restriction digestion and DNA sequence analysis.
The restriction digestion reaction mix contains,.
Seven out of eight clones had the expected restriction digestion pattern.
Res is required for restriction digestion, although it has no enzymatic activity on its own.
The presence of negative colonies generally indicates an incomplete restriction digestion of the backbone plasmid.
For example, restriction digestion can be utilized to cleave the full-length sequence at a desired location.
Orientation and sequence of the variable region gene was confirmed by restriction digestion and sequencing.
For example, restriction digestion may be utilized to cleave the full-length sequence at a desired location.
Twenty three out of twenty four transformants had the expected restriction digestion pattern.
The isolated DNA is subjected to restriction digestion by PstI and BamHI endonucleases.
Positive colonies were then screened by alkaline lysis mini-preps and BamHI restriction digestion.
Colonies were checked by colony PCR and restriction digestion prior to transfection.
Exemplary fragmentation methods include, but are not limited to sonication, mechanical shearing, and restriction digestion.
Correct clones are selected based on specific restriction digestion and DNA sequencing of the inserts.
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Is material after it has been stabilized in a digestion process
Spinach improves digestion by facilitating intestinal transit
