Examples of 'restriction digest' in a sentence
Meaning of "restriction digest"
restriction digest: A technique used in molecular biology to cut DNA into smaller fragments at specific restriction sites
How to use "restriction digest" in a sentence
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restriction digest
Positive clones were selected by restriction digest analysis.
An aliquot of the restriction digest is analysed by electrophoresis on agarose gels.
Plasmids produced were verified by diagnostic restriction digest.
An aliquot of the restriction digest is analysed by electrophoresis on agarose gel.
Insert size is determined by restriction digest.
Restriction digest and ligation reactions to clone the variable regions.
Recombinant plasmids were screened by restriction digest and confirmed by sequencing.
The restriction digest obtained can then be analyzed by a variety of means.
Eight clones had the expected restriction digest pattern.
Restriction digest analysis can provide a crude measure of the size of a triplet repeat region.
Clones were analyzed by restriction digest.
The restriction digest approach requires several extra stages of plasmid DNA manipulation.
Clones containing the desired plasmid were identified by restriction digest analysis.
Clones containing the correct restriction digest patterns are sequenced using standard procedures.
The completeness of all plasmids described above was tested by restriction digest.
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All constructs were initially screened by restriction digest and further verified by sequencing.
The proper orientation of the insert was verified by a diagnostic restriction digest.
This procedure is based on an initial restriction digest using two different restriction enzymes.
Appropriate orientations for subsequent subcloning were determined by restriction digest analysis.
These two halves are combined by restriction digest in order to produce the entire construct.
Twelve cDNAs were isolated and separated into four groups by restriction digest analysis.
Mutants were screened by restriction digest and confirmed by DNA sequencing.
The orientation of the insert was then checked by restriction digest mapping.
Orientation was determined by restriction digest and confirmed by DNA cycle sequencing.
Captured biotinylated DNA could be removed completely of the beads by restriction digest.
Plasmid clones were isolated and analyzed by restriction digest and agarose gel electrophoresis.
Plasmid DNA was prepared from the transformants and was analyzed by restriction digest.
Initial screening was by restriction digest of plasmid DNA.
Plasmid DNA from colonies transformed with the ligation were screened by restriction digest.
Epo analogs were screened by restriction digest on mini DNA preps.
The presence and identity of the plasmids was confirmed by DNA miniprep and restriction digest.
The VI encoding sequence was generated by restriction digest from an expression vector.
DNA is collected from cells and cut into small pieces using a restriction enzyme a restriction digest.
The selected clones were identified by restriction digest map and sequencing.
No restriction digest of the DNA fragments after PCR is necessary.
All ten clones had the expected restriction digest pattern.
Restriction digest to estimate the repeat length of the transfected constructs ( upper panel ).
Seven out of eight clones had the expected restriction digest pattern.
A primer can be purified from a restriction digest by conventional methods, or can be chemically synthesized.
The right construct can be isolated and checked by a restriction digest with BglII.
A restriction digest was performed on these plasmids, which showed all predicted sites to be present.
The presence of the appropriate insert was verified by diagnostic restriction digest and gel electrophoresis.
Performing a restriction digest of the DNA with a panel of restriction enzymes ;.
The beads were washed and resuspended in buffer and a restriction digest with NcoI was performed.
Performing a restriction digest of the bound ligation product of step ( h ),.
Successful recombination was verified by a Sall restriction digest of the plasmid.
Bam HI restriction digest for detection of BamHI - ANXA5 promotor allele.
Sequence and orientation were confirmed by restriction digest profiles and DNA sequencing.
Plasmid pGG17 was isolated and confirmed by sequence analysis and restriction digest.
Positive clones were identified by Alw NI restriction digest and sequence verified again.
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Let him digest that last one
We are shy one brief in the metro digest
Just let me digest this for a second
