Examples of 'reverse primer' in a sentence
Meaning of "reverse primer"
Reverse primer: In genetics and molecular biology, a reverse primer is a single-stranded piece of DNA used during PCR (polymerase chain reaction) to initiate DNA synthesis in the reverse direction
How to use "reverse primer" in a sentence
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reverse primer
The reverse primer incorporated a SphI restriction site.
A typical reaction involves a forward and a reverse primer.
The reverse primer incorporates a BamHI restriction site.
Each sample is barcoded with a unique reverse primer index.
The reverse primer incorporates a SphI restriction site.
The procedure for designing the reverse primer is described below.
The reverse primer incorporates a BamHl restriction site.
Each pair of primers comprises a forward primer and a reverse primer.
The reverse primer incorporated a BamHl restriction site.
The positions of the forward primer and reverse primer are indicated.
The reverse primer incorporated a BamHI restriction site.
The one base overlap with the reverse primer is shown below.
The reverse primer includes a complementary universal primer sequence.
At least one of either the forward or the reverse primer may be labelled.
The reverse primer was biotinylated.
See also
This enables the hybridization of the specific reverse primer to the cDNA.
Reverse primer read represented as reverse complement to aid visualization.
This can be achieved by using different ratio of forward primer and reverse primer.
The final reverse primer would be the reverse complement of this.
The mutation sites should be close to the forward primer or the reverse primer.
The reverse primer incorporates AscI and PstI restriction sites.
The spacer region may further contain the sequence of the reverse primer.
The reverse primer and detectable oligonucleotide are based on consensus sequences shared between reactions.
The universal primer pair includes a forward primer and optionally a reverse primer.
Neither the forward nor the reverse primer need contain a detectable label in such a system.
The dotted lines indicate the sequence added if a tailed reverse primer is used.
The reverse primer has a complementary sequence to the other strand of the nucleic acid sequence.
It is understood that the methods comprise at least one forward primer and a reverse primer.
The locations of the reference forward primer and reverse primer are indicated by shaded boxes.
The reverse primer incorporates a Sphl restriction site.
The nucleotide sequence of the reverse primer corresponds to nucleotide Nos.
The reverse primer incorporated a Sphl restriction site.
It is also possible to insert a promoter for RNA polymerase into the reverse primer.
The reverse primer incorporated the Hpai restriction site.
Also included in the reaction is a conventional reverse primer to create a PCR primer pair.
Nucleic acid Reverse primer for amplification of the cDNA sequence.
The first PCR amplification was completed using an outer forward primer and a reverse primer.
The reverse primer incorporated AscI and Pstl restriction sites.
The PCR was performed using each forward primer in conjunction with a relevant reverse primer.
The reverse primer hybridizes to the top end of the DNA.
In some embodiments both the forward primer A and the reverse primer have a counter sequence.
Nucleic acid Reverse primer to detect alternative splicing of the gene.
AluRev is the reverse primer.
Nucleic acid Reverse primer for amplification of the genomic sequence.
F stands for forward primer and R stand for reverse primer.
The reverse primer adds an EcoRl cloning site after the stop codon of the Fc.
The underlined portion in the reverse primer is a Bgl II site for cloning.
The reverse primer contained also an AseI restriction enzyme site and a FRT site.
An Eco RI site is also introduced into the reverse primer beside the Spel site.
The reverse primer incorporates Ascl and Pstl restriction sites.
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Examples of using Primer
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The primer includes the following modules
It was mostly gray primer and fender fill
A primer is not necessary for small repairs
Examples of using Reverse
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Proceed in reverse order to remove the accessory
This option does not work in reverse rotation
Reverse the procedure to reinstall the divider