Examples of 'reverse primers' in a sentence
Meaning of "reverse primers"
reverse primers - short nucleotide sequences used in molecular biology to initiate DNA synthesis in the reverse direction
How to use "reverse primers" in a sentence
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reverse primers
Thin lines represent forward and reverse primers.
Both forward and reverse primers are designed as described above.
These clones were sequenced using universal and reverse primers.
Reverse primers are located in the constant regions of heavy and light chains.
Horizontal arrows indicate location of forward and reverse primers.
The various forward and reverse primers that were used are described below.
An oval indicate insertion of LNAs in forward and reverse primers.
The forward and reverse primers are colored dark and light gray respectively.
In certain embodiments the forward and reverse primers may be identical.
The forward and reverse primers each hybridize to a different sense or antisense strand.
The probe anneals to specific nucleotides between the forward and reverse primers.
Forward and reverse primers overlap each other according to the diagram in fig.
The probe sequences were designed based upon the forward and reverse primers used.
Useful sets include pairs of forward and reverse primers optionally teamed with sequencing primers.
This is because different colored dyes can be used for the forward and reverse primers.
See also
Underlined are forward and reverse primers forthe SSR amplification.
The two capture primers contain sequences that correspond to the forward and reverse primers.
The forward and reverse primers are designed to amplify all or part of a CRISPR region.
Positive clones will be identified and sequenced with the commercially available forward and reverse primers.
VH and VI forward and reverse primers were as described above.
Positive clones were amplified by PCR using universal λ forward and reverse primers.
The sequences for the forward and reverse primers for Fragment A was as follows.
PCR were performed with different to combinations of these forward and reverse primers.
These primers will also serve as the reverse primers that drive PCR DNA amplification.
If two reverse primers are used, then a mixture of two reverse primer sequences is used.
Refers to sense primers and I refers to reverse primers.
The forward and / or reverse primers can be hairpin primers.
In other embodiments, only on of the forward or reverse primers is tagged.
Unlabeled probe and reverse primers were designed to be matched with wild-type.
In certain embodiments, both forward and reverse primers are tagged.
Preferably, the forward and reverse primers do not comprise overlapping nucleotide sequences.
The following is a list of the respective forward and reverse primers used,.
In one embodiment, dNTPs, ddNTPs or reverse primers are labeled with radioisotopes.
Pre-amplification of the cDNA can be accomplished using universal forward and reverse primers.
In another embodiment, dNTPs, ddNTPs or reverse primers are labeled with fluorescent agents.
A forward primer may, for example, be used to amplify fragments between two reverse primers.
Table 1 shows the forward and reverse primers used for each amplification.
Preferably, the second amplification reaction is performed using forward-internal and reverse primers.
Once the nucleic acids are prepared, forward and reverse primers are introduced to the sample.
Most preferably, the first nanoparticles contain on their surface both forward and reverse primers.
In some embodiments, the first and second reverse primers comprise the first and second labels.
In other embodiments, the multiplex method utilizes different forward primers, but the same reverse primers.
In one more preferred embodiment, dNTPs, ddNTPs or reverse primers are labeled with biotin.
Where a third initial reaction was required, the reaction contained mutagenic forward and mutagenic reverse primers.
The pin II forward and reverse primers were as follows,.
The reverse primers used for the amplification of different CBDmut products are described in Table 6.
For each region of the chromosome, a set of forward and reverse primers is designed.
Gene-specific reverse primers used in amplification of sequences in the first PCR reaction.
Solutions of the murine Y or k constant region reverse primers were also prepared at 50µM.
Gene-specific reverse primers were used in amplification of sequences in the 2nd PCR reaction.
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Hybridisation of the primers with the template
Examples of using Reverse
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Proceed in reverse order to remove the accessory
This option does not work in reverse rotation
Reverse the procedure to reinstall the divider