Examples of 'scfvs' in a sentence
Meaning of "scfvs"
SCFVs stands for Single Chain Fragment Variables, which are antibody fragments used in research and diagnostics
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- plural of scFv
How to use "scfvs" in a sentence
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scfvs
The scFvs can be attached vía a linker sequence.
Chimeric whole antibodies derived from scFvs.
Bound scFvs were detected as described above.
The seven clones encode different scFvs.
Multiple scFvs can be linked in a single polypeptide chain.
Bivalent and trivalent scFvs.
None of the scFvs gave a signal above background.
Such antibodies are known as ScFvs.
Myc tag was fused to the ScFvs to enable them to be detected.
The scFvs described and characterized herein were produced as follows.
The molecular weights of all purified scFvs were checked by mass spectrometry.
Higher affinity scFvs are selected by affinity chromatography on antigen as described above.
This sequencing requires a large number of scFvs to perform a statistical analysis.
In contrast to scFvs nuclear localisation was not seen with the peptides.
Diabodies are dimeric scFvs.
See also
This proportion of intracellularly expressed scFvs is much higher than in previously described libraries.
Selected fluorescent micrographs of expression of randomly picked scFvs in HeLa cells.
Purification of scFv ScFvs containing a poly histidine tail are purified by immobilised metal affinity chromatography.
Free thiol groups are necessary for labelling scFvs with technetium.
Methods for converting scFvs into conventional antibody molecules are well known within the art.
Single domain antibodies will sometimes be preferable to scFvs for oligomerization purposes.
The triplex structure implies that each fibre will thus end in three ScFvs.
This underlines the specificity of the scFvs that can be isolated from this library.
Ten individual clones from the spleen library were sequenced and four different scFvs were found.
Therefore we tested the aggregation behavior of the scFvs at high concentrations and elevated temperatures.
This indicates the value of the filamentous phage approach for obtaining tumour targeting scFvs.
Bacterial hosts are preferably used for cloning the scFvs and for producing the scFv proteins.
ScFvs can be produced either recombinantly or synthetically.
Graphical representation of the expression of the activity of selected scFvs under reducing conditions.
ScFvs can also be obtained from a phage display library.
Next tested was whether the library contained clones expressing soluble scFvs in the periplasm.
Display of scFvs on the surface of mammalian cells fused to Fc domains.
The teaching also includes multispecific molecules comprising more than two scFvs binding domains.
It was possible to rank the scFvs with respect to their binding to EGFR.
The scFvs were tested by ELISA for binding to three tumor cell lines.
Periplasmic fractions of five FITC specific scFvs were prepared as described above.
One of the scFvs from the in vitro immunized library bound to EGFR.
Example SEC chromatograms from purified scFvs with positively charged linkers.
ScFvs ScFvs are intracellularly active molecules having binding property comparable to that of an antibody.
Supematants containing scFvs were diluted in PBSM and added to the plates.
ScFVs were purified by affinity chromatography and used for the construction of bispecific scFv.
Fractions containing scFvs were then pooled and dialyzed with PBS.
The individual VH and VI reactions must now be combined to get scFvs.
A representative proportion of scFvs from the output of selection rounds were subjected to DNA sequencing.
Intrabodies include single domain fragments such as isolated VH and VI domains and scFvs.
None of the scFvs from the spleen and lymph node library bound to EGFR.
Evaluating Identified scFvs in binding assays.
Preparation of Semisynthetic scFvs.
The cDNAs coding for the scFvs of erb were PCR amplified from the erb phagemid.
In some aspects, the antibody fragments are scFvs.
