Examples of 'scfv fragment' in a sentence
Meaning of "scfv fragment"
scfv fragment: In biology and biochemistry, this term refers to a small recombinant antibody fragment that is commonly used for research, diagnostics, and therapy purposes
How to use "scfv fragment" in a sentence
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scfv fragment
Particularly preferred is the scFv fragment format.
The scFv fragment is produced in soluble form in the supernatant.
The supernatant was tested to demonstrate the presence of a functional scFv fragment.
The scFv fragment is capable of specifically binding to free morphine.
In vivo targeting experiments in nude mice employing this scFv fragment failed.
Preferably the ScFv fragment comprises a linker sequence between light chain and heavy chain variable regions.
Preferably, said antibody fragment is a scFv fragment.
Each scFv fragment is assembled by PCR.
For in vivo uses, the antibody can be a scFv fragment.
Rotating scFv fragment with highlighted complementarity determining regions (CDRs).
Typically, the VH and VL domains in a scFv fragment are linked by a linker sequence.
The antibody fragment is preferably a humanized antibody fragment, such as Fab fragment and / or scFv fragment.
Thus, this antigen-specific scFv fragment exhibits binding characteristics and the fine specificity of a TCR-like molecule.
A clone from patient 5 was grown up in a 1L volume the scFV fragment purified from the periplasm.
An scFv fragment isolated from a semi-synthetic phage display scFv antibody library and designated C21.
See also
AL5 is an ampicillin binding scFv fragment and serves as negative control.
Brief Description of the Drawings Figure 1 shows a method of producing an scFv fragment in phage.
After induction with IPTG, the scFv fragment is produced in soluble form in the periplasma.
In one example, such as for in vivo uses, the antibody can be a scFv fragment.
The recombinant B-lactoglobulin-specific scfv fragment by phage display selection.
In some embodiments, the antigen-binding portion is Fab or scFv fragment.
Several B-lactoglobulin-specific scFv fragment clones were obtained.
In some embodiments, the NGF antagonist is an anti-NGF scFv fragment.
The diabody scFv fragment was derived from the L19 antibody.
The invention provides an isolated functional antibody scFv fragment that mimics HER-2/neu.
Binding scFv fragment was detected using the anti-FLAG antibody M1.
Furthermore, the invention also provides a stabilized anti-EGP-2 scFv fragment.
After functional evaluation, the scfv fragment was ligated to a DNA fragment encoding PE40.
The scFv fragment was purified using the HisTrap Kit ( Amersham Pharmacia Biotech, Uppsala, Sweden ).
FIG . 1 represents the polypeptide sequence of an scFv fragment derived from the antibody CD28.3.
The M79 scFv fragment was cloned as described by Mack.
Nucleic acid according to claim 11 encoding the ScFv fragment of SEQ ID No. 2.
More preferably the ScFV fragment has essentially the sequence of SEQ ID No. 2.
The amino acid sequence of anti-M1+morphine immune complex scFv fragment was named K11 scFv.
The M79 scFv fragment was cloned as described by Mack et . al.
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Examples of using Scfv
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Crude scFv supernatants were prepared as described above
Particularly preferred is the scFv fragment format
The scFv can be attached vía a linker sequence
