Examples of 'secondary antibodies' in a sentence
Meaning of "secondary antibodies"
secondary antibodies: Secondary antibodies are antibodies that specifically bind to primary antibodies. They are used in many laboratory techniques, such as Western blotting and immunohistochemistry, to amplify the signal and aid in the detection of specific proteins
How to use "secondary antibodies" in a sentence
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secondary antibodies
Secondary antibodies can be monoclonal or polyclonal antibodies.
Search for primary and secondary antibodies.
Many suitable secondary antibodies are commercially available.
Precipitation can also be obtained by means of secondary antibodies.
The secondary antibodies are typically conjugated to an enzyme or a fluorophore.
A wide variety of labeled secondary antibodies are available commercially.
Secondary antibodies linked to fluorochromes can be obtained commercially.
This indicates that both labeled secondary antibodies are bound specifically.
Secondary antibodies combined with the enzyme are added to the plate.
Bound antibodies were revealed by the appropriate fluorescently labelled secondary antibodies.
Primary and secondary antibodies were selected as described above.
The negative control used was cells reacted only with secondary antibodies.
Superfluous secondary antibodies were removed by three washing steps.
This complex can be detected either directly or indirectly by using secondary antibodies.
Multiple secondary antibodies can bind a single primary antibody.
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No fluorescence was associated with cells after incubation with secondary antibodies alone.
Incubation of the secondary antibodies was performed in the blocking solution.
No fluorescence is associated with cells after incubation with secondary antibodies alone.
These two labeled secondary antibodies are combined before use.
Unlabeled antibodies may be then contacted with the corresponding labeled secondary antibodies.
Incubation of the secondary antibodies is performed in the blocking solution.
Cells were washed twice in between incubations with primary and labeled secondary antibodies.
Molecular probes secondary antibodies were used following manufacturer recommendations.
All filters were extensively washed and appropriate secondary antibodies were incubated and washed.
Labelled secondary antibodies are binding to the haptens on the carrier molecule.
All fitters were extensively washed and appropriate secondary antibodies were incubated and washed.
Preferably secondary antibodies especially secondary antibodies which are specifically labeled.
Human antibodies are detected with peroxidase conjugated secondary antibodies directed against human immunoglobulins.
By using secondary antibodies or by labeling at least one of the binding partners.
These primary antibodies may be directly labeled or may be detected with suitable secondary antibodies.
Labelled secondary antibodies are binding to the haptens of the binding entity.
Membranes were then incubated with peroxidase conjugated secondary antibodies for one hour at room temperature.
Chemiluminescent secondary antibodies may be obtained from any number of commercial sources.
The immunological detection of bound antibodies has been carried out using fluorescence labeled secondary antibodies.
Primary and secondary antibodies also can be coupled to radioactive or fluorescent tags.
Results were similar for oocysts stained with both IgG specific and IgM specific secondary antibodies.
Secondary antibodies and substrates for colour reactions were used as described before.
This was followed by exposure to a secondary antibodies having a different fluorescent tag than the albumin.
Secondary antibodies are conjugated with Alexa dye.
Exemplary secondary ligands are those secondary antibodies that have binding affinity for the first antibody.
All secondary antibodies were polyclonal antisera raised in goat against the human proteins.
Bound antibodies were detected with HRP conjugated secondary antibodies.
Incubation with primary and secondary antibodies was carried out according to standard protocols.
The cells were washed with PBS to remove unbound secondary antibodies.
While working with secondary antibodies keep samples protected from light as much as possible.
FITC conjugated antibodies directed against mouse IgG were used as secondary antibodies.
This causes several secondary antibodies to bind to a single primary antibody leading to signal amplification.
Bertin Bioreagent offers primary and secondary antibodies for laboratories.
The primary and secondary antibodies may recognize and bind to the same or different antigens.
Increased class II antigen expression is determined by flow cytometry using the appropriate secondary antibodies.
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