Examples of 'secondary antibody' in a sentence
Meaning of "secondary antibody"
This phrase is used in the field of biology or immunology to describe an antibody that is used in certain laboratory techniques or experiments. It refers to an antibody that binds specifically to another primary antibody, allowing for the detection, visualization, or purification of specific molecules or cells within a sample
How to use "secondary antibody" in a sentence
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secondary antibody
Primary and secondary antibody deficiency disorder.
A package can also contain a secondary antibody.
Primary and secondary antibody deficiency disorders.
Control femurs were incubated in secondary antibody only.
Secondary antibody alone is shown as negative control.
None designates that no secondary antibody was used.
The secondary antibody can be detectably labeled.
Characteristics of the primary and secondary antibody responses.
Also the secondary antibody may be labeled.
Luciferase enzyme NanoLuc was fused to secondary antibody.
A secondary antibody provides an example of indirect coupling.
Control samples were stained with secondary antibody alone.
This secondary antibody is chemically linked in advance to an enzyme.
The source of the secondary antibody.
The secondary antibody is also used at saturating concentration.
See also
Negative controls included omission of primary or secondary antibody.
Secondary antibody was not added to the isotype controls.
An enzyme is conjugated to this secondary antibody.
Secondary antibody alone was included as a control.
The primary antibody and the secondary antibody were as follows.
The secondary antibody was radiolabeled to allow immunoblot detection.
A fluorescently labelled secondary antibody.
A secondary antibody was dropwise added thereto.
The tissue sections were incubated in the secondary antibody as follows.
The secondary antibody is typically polyclonal.
The detectable label can be conjugated to the secondary antibody.
A suitable secondary antibody coupled to a fluorescent marker was then used.
Wells were washed as described above to remove unbound secondary antibody.
A secondary antibody conjugated to peroxydase will allow the visualization.
Antibodies can be detected by the use of a labeled secondary antibody.
Secondary antibody alone or cells only are shown as negative controls.
Blot was developed using secondary antibody conjugated to alkaline phosphatase.
All these antibodies may therefore be recognized by a single secondary antibody.
The secondary antibody can be prepared according to a known method.
Antibody incubation and detection with secondary antibody were described above.
A labelled secondary antibody is added to the wells and incubated.
Magnetic beads that are covalently coupled to a secondary antibody are commercially available.
A labeled secondary antibody is added to the wells and incubated.
Antibodies that bind to the antigen are detected by a specific secondary antibody.
The secondary antibody will be aspirated from the wells.
Biotinylated goat anti mouse immunoglobulin used as a secondary antibody was added.
The secondary antibody is properly labeled to allow immunoblot detection.
An isotype control antibody and secondary antibody alone were used as negative control.
A secondary antibody may alternatively be labeled with a chemiluminescent marker.
Antibodies of the IgG class are predominantly active during a secondary antibody response.
A secondary antibody is then also added to detect the antigen.
Captured polypeptide variants were detected by adding a primary and then a secondary antibody.
A secondary antibody also can be labeled with a chemiluminescent marker.
Plural molecules of biotin are preliminarily conjugated to each molecule of the secondary antibody.
The secondary antibody in such a case will be coupled to a detectable label.
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