Examples of 'sequencing library' in a sentence
Meaning of "sequencing library"
sequencing library - This phrase refers to a collection of ordered genetic sequences or fragments used in biological research, particularly in the field of genomics. It involves organizing and analyzing DNA or RNA sequences to study genetic variations or gene expression patterns
How to use "sequencing library" in a sentence
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sequencing library
A target sequencing library was prepared as follows.
The fragmentation method is a key aspect of sequencing library construction.
A clonal indexed sequencing library of the amplified product can be generated.
The method comprises sequencing a polymorphic target nucleic acid sequencing library as follows.
Also disclosed is a sequencing library prepared by any one of the foregoing methods.
The hybrid capture method introduces fewer errors into the final sequencing library as a result.
A sequencing library is prepared from naturally fragmented or forcibly fragmented DNA.
Obtaining a sequencing library.
Figure 5 shows a schematic of fragments of the sequencing library.
In some cases, a sequencing library is prepared prior to or during a sequencing process.
No gaps, despite using a single sequencing library.
The contiguously-linked sequencing library can be sequenced to derive contiguous sequence information.
Combine libraries in equimolar amounts ( or as desired ) for multiplexing of sequencing library.
Subsequent to isolation of the nucleic acids, a sequencing library may be obtained as described above.
In some embodiments, the extended nucleic acids provide templates for a sequencing library.
See also
Preparing a second sequencing library of said amplified polymorphic target nucleic acids ; and.
This will result in a contiguously-linked sequencing library with unique barcodes.
Preparing a first sequencing library of said mixture of fetal and maternal nucleic acid molecules ;.
In some embodiments, methods for generating a next-generation sequencing library are provided.
Use of the sequencing library according to claim 5 as a library for a high throughput sequencing platform.
The claimed invention involves methods for generating a next-generation sequencing library.
Two mismatches were allowed ; complexity of the sequencing library was significantly reduced after target enrichment.
Accordingly, the present invention provides an ultra-fast method for preparing a sequencing library.
SNPs, and prepare a target sequencing library as follows.
In some embodiments, the methods further comprise amplifying the next-generation sequencing library.
A primary sequencing library was prepared using purified cfDNA as described in Example 1.
Figure 24 shows an exemplary scheme of assembling the sequence information from clonal indexed sequencing library.
Sequencing of the sequencing library is accomplished by next generation sequencing ( NGS ) methods.
In some embodiments, methods for one-sided transposition can be used for preparing a sequencing library.
A target sequencing library was prepared from a portion of the purified cfDNA as described in Example 2.
The use of these Y-adapters ensures that potentially every fragment can become a sequencing library.
Figure 15 Comparison of sequencing library preparations obtained using Tn5 transposase and an in-house prepared transposase.
Generating a next-generation sequencing library by,.
Accordingly, the present invention relates to, inter alia, a method for preparing a sequencing library.
FIG . 5 shows a schematic of fragments of the sequencing library.
Further, 5 ' end of the backward extended primers contains linker sequences for high throughput sequencing library.
Step 3, Preparing the plasma DNA into a sequencing library.
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