Examples of 'sequencing reaction' in a sentence
Meaning of "sequencing reaction"
sequencing reaction - This phrase involves the process of determining the order of nucleotides in a DNA or RNA molecule
How to use "sequencing reaction" in a sentence
Basic
Advanced
sequencing reaction
A sequencing reaction is performed on the isolated plasmid.
Further optimization of the sequencing reaction may be required.
Sequencing reaction products provides an alternative to probing.
The following sequences are obtained from a sequencing reaction.
The sequencing reaction mix is cleaned up by ethanol precipitation.
Some of these improvements have related to the sequencing reaction itself.
A prepared sequencing reaction mastermix was aliquoted into separate tubes.
The one unprotected primer is elongated in a sequencing reaction and a sequence is determined.
The sequencing reaction can then be started by adding these metal ions.
The technology relates to minimizing sequence errors in amplicons as detected by a sequencing reaction.
Sequencing analysis and sequencing reaction were performed as mentioned above.
This dramatically reduces errors due to miscalling during the sequencing reaction.
Continue the sequencing reaction by adding the next complementary base.
Heat inactivation can be useful to stop a sequencing reaction set forth herein.
An ideal sequencing reaction will produce bands of uniform intensity throughout the gel.
See also
Sequence information may be obtained by conducting one or more cycles of a sequencing reaction.
The fourth step involves performing a sequencing reaction simultaneously on a plurality of the reaction chambers.
The enriched target sequence may be subjected to a primer extension sequencing reaction.
A single base sequencing reaction using labeled dideoxynucleotide terminators may be used to detect the variation.
Current sequencing technologies require millions of copies of nucleic acid per sequencing reaction.
In these systems a single chain termination sequencing reaction mixture is run per lane.
A sequencing by synthesis reaction can alternatively be based on a terminator dye type of sequencing reaction.
The data analysis method makes use of a standard sequencing reaction which is performed as follows.
The primers are selected so that each produces a distinct set fragments in the sequencing reaction.
A second sequencing reaction can subsequently be performed using this newly generated strand as a second template.
An apparatus comprising a sequencing module may perform a sequencing reaction known in the art.
Other methods of deconvoluting sequencing reaction mixtures include the use of gas phase ion spectrometry.
Sequencing by synthesis reactions can also comprise a terminator dye type sequencing reaction.
The primers used for the sequencing reaction were the same as for the amplification step.
Each strand of the adapter ligated amplicon may serve as a template for a sequencing reaction.
The sequencing was done using a Sanger sequencing reaction followed by capillary electrophoresis.
Colonies containing the clone were confirmed via restriction enzyme digestion and sequencing reaction.
The photons generated by the pyrophosphate sequencing reaction are captured by the CCD.
The sequencing reaction begins by introducing a DNA polymerase and a fluorescently labeled nucleotide.
DNA template was added to the sequencing reaction mix.
This sequencing reaction will read through the MID and desired site in the insert.
Oligonucleotide RNS served as a primer for the sequencing reaction.
The sequencing reaction is carried out using the Applied Biosystems kit ref.
The fragments are then amplified by PCR and the sequencing reaction is carried out.
Preferably, such a sequencing reaction is a sequencing by synthesis reaction.
Figure 15 illustrates transfer of barcoded tagged molecules to a sequencing reaction.
Performing a sequencing reaction that comprises repeated cycles of,.
Such strategy can be used in single-molecule sequencing reaction.
When this sequencing reaction is performed, errors can occur.
After purification of pcr products, the fragments were subjected to sequencing reaction.
Preferably, cycles of sequencing reaction and data analysis are performed concurrently.
Thus, following detection of the product, a subsequent sequencing reaction is unnecessary.
Finally, a sequencing reaction is performed simultaneously on a plurality of the reaction chambers.
Each reaction included 2 µl of the sequencing reaction mix and 3 µl of diluted template.
A cycle sequencing reaction was performed following the manufacturer 's recommended procedure.
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It would give its reaction at a later date
Quick reaction times in case of delay s or malfunctioning
I am having a physical reaction to how bad this is
Examples of using Sequencing
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Appropriate sequencing of regulatory consolidation and liberalization
There was discussion regarding the appropriate sequencing of
The process and sequencing of reform were emphasized
