Examples of 'shrnas' in a sentence
Meaning of "shrnas"
Shrnas: This term does not appear to have a widely recognized definition or common usage in English
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- plural of shRNA
How to use "shrnas" in a sentence
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shrnas
Two validated shRNAs were used as positive controls.
Typically the medicament may comprise a pair of shRNAs.
A general toxicity control of shRNAs included normal mouse bone marrow cells.
Hairpin dsRNAs are typically referred to as shRNAs.
Lentiviral vectors expressing scrambled shRNAs were also produced to be used as negative control.
A variety of promoters have been used to drive the expression of shRNAs.
Stable cell lines expressing shRNAs were generated by retroviral mediated gene transfer.
Structurally designed shRNAs.
The robust expression of optimized shRNAs may be desirable for certain applications.
Such guide sequences were delivered into the cells in the form of shRNAs.
Design of new shRNAs to mimic mature guide strands produced in pLKO vector.
Firefly luciferase could also be specifically silenced by cotransfection with homologous shRNAs.
The virus expresses high levels of shRNAs and is very efficient in gene silencing.
Inhibition of cervical cancer cell growth in vitro and in vivo with dual shRNAs.
We therefore also designed shRNAs that paired imperfectly with their target substrates.
See also
Several groups have studied the use of viral vectors to introduce shRNAs into organisms.
Reports that shRNAs expressed from plasmids could trigger RNAi allowed the use of viral vectors.
Also included in the invention is an isolated mammalian cell comprising the shRNAs described herein.
ShRNAs are more stable than synthetic siRNAs.
Mature shRNA sequence distributions were calculated for each shRNA before averaging across shRNAs.
ShRNAs targeting sequences across the huntingtin gene.
We therefore tested whether shRNAs were substrates for the Dicer enzyme.
ShRNAs can be abundantly expressed to provide efficient knockdown.
Northern blot analysis of total RNA extracted from cells transduced with multiple shRNAs and shRNAmiRs.
ShRNAs of the disclosure include the sequences of the desired siRNA duplex.
Constructs for lentivirally delivered shRNAs were obtained from Sigma as MISSION glycerol stocks.
ShRNAs contain a stem loop structure.
When the total RNA from the cell line containing shRNAs to SEQ.
ShRNAs targeting XL isoform alone have strong effect on eY induction.
However, silencing can also be triggered by endogenous expression of shRNAs.
High levels of shRNAs are obtained with RNA pol III promoters.
Furthermore, we have validated delivery of shRNAs using retroviral vectors.
SiRNA and shRNAs knockdown Pooled siRNAs samples were obtained from Dharmacon.
This made it possible to produce in transfected cells shRNAs with the sequence,.
Other shRNAs were cloned using a tailed-PCR strategy.
Knock-down of gene expression can be directed by the use of siRNAs or shRNAs.
Other shRNAs were cloned using a tailed-PCT strategy.
Nonlimiting examples of siRNAs and shRNAs of this disclosure are provided in Table 2.
Overall, shRNAs were somewhat less potent silencing triggers than were siRNAs.
As had been observed in S2 cells, shRNAs were effective inducers of gene silencing.
The shRNAs are double-stranded RNAs built to present a similar structure to miRNAs.
Construction of replication competent H-1PV for the delivery and expression of shRNAs.
Modified shRNAs can retain the modifications in the post-Dicer processed duplex.
Exemplary RNA silencing agents include dsRNAs such as siRNAs, miRNAs and shRNAs.
In vivo, shRNAs may cause toxicity in mouse striatum independent of HDh mRNA silencing.
PHEM were transduced with the lentiviral vectors expressing the shRNAs MyoVa exF n o, 2.
By contrast, vector-expressed shRNAs can suppress gene expression over prolonged periods.
ShRNAs are single-stranded polynucleotides with a hairpin loop structure.
Tissue-specific, inducible expression of shRNAs involves truncation of polII promoters to the transcription start site.
ShRNAs are constantly synthesized in host cells, leading to more durable gene silencing.
