Examples of 'spin column' in a sentence
Meaning of "spin column"
A 'spin column' is a laboratory device used for separating components of a mixture based on their size or other properties through centrifugal force
How to use "spin column" in a sentence
Basic
Advanced
spin column
The filter is contained in spin column.
Spin column purification can be used to remove free nucleotides.
Pelleted resin was resuspended and transferred to a spin column.
Centrifugation of the spin column results in expression of the void volume.
Both sense and antisense strands are isolated by spin column chromatography.
Put a spin column in a new Falcon tube.
The eluent was collected and the spin column was discarded.
The modified oligonucleotides are purified from the free nucleotides on a spin column.
Only the aqueous phase was applied to a spin column to remove protein contaminants.
The digests were purified using a Qiagen spin column.
PCR products are purified using a spin column to remove excess primers from the previous PCR.
Released miRNA can then be purified by precipitation or using spin column chromatography.
The mix was bound to QIA Spin column and washed twice with PE buffer.
The DNA probe was gel purified using a spin column.
Keep the Spin Column in this step.
See also
Labelled DNA was separated from unincorporated label by spin column chromatography.
Place a QIAquick spin column in a provided 2 ml collection tube.
DNA is extracted from vector particles using a commercially available ion exchange spin column.
RNA purification is preferably conducted on a spin column containing silica or other material.
The PCR fragment was purified with a Qiagen spin column.
The spin column was placed into a new 2 mL collection tube.
The DNA probe was gel purified using a spin column as disclosed above.
Isolated single colonies were selected and plasmid was then purified using a QIAGEN Spin Column.
Typically, five minutes on a spin column gives sufficient purification.
Each sample-resin mix was transferred to an empty spin column.
A spin column was placed in a 5 mL tube and the purification resin was stirred.
Discard the RNeasy Mini spin column.
Close each spin column to avoid cross-contamination during centrifugation.
Pipet the mixture from step 6 into a spin column.
The spin column is now ready for purifying the conjugated antibody ;.
In some embodiments, the centrifuging comprises centrifuging through a spin column.
Place the RNeasy MinElute spin column into a new 2 ml collection tube.
In some examples, the centrifuging comprises centrifuging through a spin column.
When loading spin columns, ensure that spin column hinges are not pointed outwards.
Preferably, the capture surface is housed in a spin column or vacuum system.
Spin column purification and / or magnetic bead technology and reversed-phase purification are frequently applied.
Extracts were gently resuspended and loaded onto a QIAquick ™ spin column.
Nucleic acid purification techniques can include spin column separation, precipitation, and electrophoresis.
Optionally, remove digestion products from desired fragments with a spin column.
The probe was purified using a Bio-Rad chromatography spin column according to the manufacture 's instruction.
Each sample was vortexed, briefly spun down, and transferred to a MinElute spin column.
A labelling kit typically contains a ready-to-use spin column for purification of a biotinylated probe from excess reagents.
For small biological samples, preferably a size selection spin column is used.
The desulfonation reaction using a spin column was performed as described above in Example 5.
In a preferred embodiment, the capture surface is housed in a spin column or vacuum system.
A single spin column from the Qiagen Min-Elute kit was used.
These oligonucleotides phosphorylated at the 5 ' end are purified from the free nucleotides on a spin column.
Purification of a conjugate can be easily accomplished using a spin column or a size-exclusion spin column.
The DNeasy Mini spin column is then placed into a new 2 ml collection tube.
Repeat step 7 until the whole sample has been pipetted into the spin column.
You'll also be interested in:
Examples of using Spin
Show more
Low speed spin helps reduce wrinkling
The washing machine does not drain or spin
We have to spin this before it gets spun
Examples of using Column
Show more
This column also captures those situations
This marks my final column in this magazine
A column matrix is a matrix with a single column
