Examples of 'spin columns' in a sentence
Meaning of "spin columns"
Spin columns: This term refers to a laboratory technique used in the purification or isolation of DNA, RNA, proteins, or other biomolecules. Spin columns contain membranes or resins that separate biomolecules based on specific properties like size, charge, or affinity, enabling their purification through centrifugation
How to use "spin columns" in a sentence
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spin columns
Higher rotor lid to accommodate spin columns.
The spin columns were used as recommended by the vendor.
Alternatively one can use commercially available spin columns.
Spin columns typically contain a filter material with a silica surface.
The current preferred method of sample purification makes use of spin columns.
For example, a series of spin columns comprising different adsorbents can be used.
Excess DTT can be removed by size exclusion chromatography using spin columns.
RNA extraction and binding to spin columns is done using the manufacturer 's guidelines.
DNA fragment purification was performed using QIAGEN spin columns.
Desalting spin columns are widely available with various volumes and MWCO limits,.
The genomic DNA is removed from the samples by using gDNA Eliminator spin columns.
Supernatants were concentrated on spin columns with cut-off size of 10kDa.
Radiolabelled oligonucleotide was separated from unincorporated nucleotides using Sephadex G50 spin columns.
Two MinElute spin columns ( Qiagen ) were used for purification.
The labeled cRNA was purified using spin columns e . g.
See also
Plasmid DNAs were purified using QIAprep spin columns ( QIAGEN ) according to manufacturer 's instructions.
Accessories for accommodating additional tubes e.g. stool tubes or spin columns on request.
The cleaved DNA was purified using QiaQuick spin columns ( Qiagen ) according to the manufacturer 's recommendations.
ROTORS AND ACCESSORIES Additional tubes e.g. stool tubes or spin columns on request.
The PPP was purified via Ni-NTA Spin Columns ( Qiagen ) as recommended in the manual.
Optionally, the kit may further comprise pre-fiaotionation spin columns.
The fractions were then concentrated using 4ml spin columns ( Agilent ) with a 5kDa molecular weight cut-off.
There are many commercial products available for accomplishing this, such as, for example, spin columns.
Figure 10A illustrates the effect of spin columns on uptake of ciprofloxacin at 60 °C.
The enlarged lid for the rotor, MSL-SC, allows centrifuging spin columns.
The biotinylated proteins were buffer-exchanged using protein desalting spin columns from Pierce equilibrated in TBS.
Nucleotides were removed by phenol / chlorofarm extraction and Sephadex G50 spin columns.
Additional tubes e.g. stool tubes or spin columns on request.
Nucleotides were removed by phenol / chloroform extraction and Sephadex G50 spin columns.
Removal of excess of DyeTerminators were performed using Quick Spin columns ( Boehringer Mannheim ).
Phosphate buffer was then removed by centrifugation over 2 ml Sephadex ® G50 spin columns in water.
In this respect plasmid DNA was purified with QIAprep Spin columns ( Qiagen ).
The probes were separated from unincorporated nucleotides by passage through Sephadex G-50 mini spin columns ( Boehringer ).
The eluate was desalted by passage through Sephadex G-25 spin columns.
The 2428 rotor was specially developed for the centrifugation of spin columns.
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