Examples of 'stably transfected' in a sentence
Meaning of "stably transfected"
stably transfected: A term used in molecular biology to describe cells that have had a piece of foreign DNA integrated into their genome in a stable manner, allowing for continuous expression of the inserted gene
How to use "stably transfected" in a sentence
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stably transfected
Sorting of stably transfected cells by flow cytometry.
The cells can thus be transiently or stably transfected.
Stably transfected cells were obtained as described above.
Also provided are stably transfected cell lines.
Stably transfected clones were isolated.
To use antibiotic selection markers to select stably transfected clones.
Cells are stably transfected to express these fusión proteins.
Maintenance of cells and development of stably transfected cell lines.
The cells were stably transfected with the vector alone.
The detection of expression can be done in transiently or stably transfected mammalian cell.
Was stably transfected.
The reporter construct is typically transiently or stably transfected into a cell.
Four stably transfected clones of each construct were selected for further analysis.
The antibiotic resistant colonies are pooled to yield the stably transfected cells.
Techniques for establishing stably transfected microorganisms are known in the art.
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This plasmid is desirably constructed so that it may be stably transfected into a cell.
Selection of stably transfected clonal cell lines exhibiting high sensitiveity by serial dilution.
Respective systems are also useful to generate and identify stably transfected clones.
An alternative to stably transfected packaging cell lines is to use transient transfected cell lines.
Compounds of interest may be administered to the stably transfected cells alone or in combination.
Cells stably transfected with the introduced nucleic acid can be identified by drug selection.
The host cells can be transiently or stably transfected with the recombinant expression vector.
Such properties adversely affect the rate of transfection and impede selection of stably transfected cells.
For each construct four stably transfected clones were expanded and chosen for further analysis.
The expression vectors may also contain a selectable marker for propagation in stably transfected cells.
Stably transfected cells were selected by Zeocin resistance.
I cells carried in RPMI were stably transfected with pSURslaml.
Stably transfected cells were selected using Hygromycin.
Subsequently the constructs were stably transfected in BHK cells.
Stably transfected EPO producing cells are selected according to their resistance to geneticin.
The FVIII mutant produced may be stably transfected into cells.
Clones of stably transfected cells are then screened for expression of activated protein C.
Cells are a useful system to study HBV production in a stably transfected environment.
Expression of EGFR in stably transfected cells was confirmed by immunoblotting.
Successive changes of medium are performed to screen for stably transfected CHO cell clones.
Cells were transiently or stably transfected with a plasmid encoding Renilla luciferase.
In one embodiment of the invention the myeloma cells are stably transfected with a DNA molecule.
All pNATHA were stably transfected in a HeLa Tet Off cell line.
The engineered immunoglobulin expression vector may then be stably transfected into CHO cells.
Stably transfected and chimeric antibody-producing cells were cloned.
The plasmids may also be transiently or stably transfected into CHO cells.
Contacting said stably transfected cells with said compound of interest ; and.
In still other embodiments, transfected cells are stably transfected.
No selection marker was used and the stably transfected cells were selected by FACS staining.
In a first step, the repressor protein expression vector is stably transfected.
Briefly, an aliquot of supernatant from stably transfected cells is transferred to a clean microplate.
Stably transfected cells are seeded onto a 35 mm culture dish containing a glass coverslip insert.
We purified protein from stably transfected CHO cells.
Stably transfected cells were seeded onto a 35 mm culture dish containing a glass coverslip insert.
In another embodiment, the cells are stably transfected with nucleic acids.
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Examples of using Transfected
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Transfected with human hepatic cancer cells
Thereby successfully transfected cells can be selected
A transfected cell can also serve as a carrier
Examples of using Stably
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Outputted stably and meeting medical standards
The steriliser may not be stably positioned
Sorting of stably transfected cells by flow cytometry
