Examples of 'stably transfected cells' in a sentence
Meaning of "stably transfected cells"
stably transfected cells - This phrase refers to cells that have been genetically modified to permanently incorporate foreign genetic material through stable transfection techniques. These cells can stably express the introduced genes over multiple cell divisions, allowing for consistent gene function and protein production
How to use "stably transfected cells" in a sentence
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stably transfected cells
Sorting of stably transfected cells by flow cytometry.
The antibiotic resistant colonies are pooled to yield the stably transfected cells.
Stably transfected cells were obtained as described above.
Compounds of interest may be administered to the stably transfected cells alone or in combination.
Stably transfected cells were selected by Zeocin resistance.
Such properties adversely affect the rate of transfection and impede selection of stably transfected cells.
Stably transfected cells were selected using Hygromycin.
The expression vectors may also contain a selectable marker for propagation in stably transfected cells.
Expression of EGFR in stably transfected cells was confirmed by immunoblotting.
Two complementing fragments of the b-galactosidase enzyme are expressed within stably transfected cells.
No selection marker was used and the stably transfected cells were selected by FACS staining.
Stably transfected cells are seeded onto a 35 mm culture dish containing a glass coverslip insert.
Briefly, an aliquot of supernatant from stably transfected cells is transferred to a clean microplate.
Stably transfected cells were seeded onto a 35 mm culture dish containing a glass coverslip insert.
To summarize, most clones derived from stably transfected cells with PCMV seemed to be inactive.
See also
Stably transfected cells were selected by culturing in Schneider 's medium containing geneticin.
Puromycin was then added at a concentration of 10 µg / ml to select for stably transfected cells.
Clones of stably transfected cells are then screened for expression of activated protein C.
Figure 5 shows the pool productivity of the stably transfected cells of Figure 4.
Contacting said stably transfected cells with said compound of interest ; and.
After two months of 1:5 splits, a pool of stably transfected cells was obtained.
A population of stably transfected cells is clonally identical, being derived from a single transfected progenitor cell.
Stably transfected cells were selected by adding 25 µg / ml blasticidin over several weeks.
Stably transfected cells were seeded into 96-well microtiter plates and cultured until confluent.
Stably transfected cells were grown in T75 culture flasks for 36 hours before each experiment.
Stably transfected cells were selected with 250µg hygromycin.
Stably transfected cells were selected for 1 week in growth medium containing 1mg . cm-3 Geneticin.
Stably transfected cells were selected by their resistance to G418.
Stably transfected cells were selected by growth in G418 medium.
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Examples of using Transfected
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Transfected with human hepatic cancer cells
Thereby successfully transfected cells can be selected
A transfected cell can also serve as a carrier
Examples of using Cells
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For cells or batteries packed with equipment
They were beaten in the cells or in the courtyard
The cells we want can not be tainted
Examples of using Stably
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Outputted stably and meeting medical standards
The steriliser may not be stably positioned
Sorting of stably transfected cells by flow cytometry
