Examples of 'step gradient' in a sentence
Meaning of "step gradient"
step gradient - a term used in mathematics or science to describe a gradual change in steps or levels
How to use "step gradient" in a sentence
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step gradient
Step gradient salt elution was applied to the column.
Columns were generally run as a step gradient.
A step gradient can be used to separate the peptide composition.
An alternative to using a linear gradient is to use a step gradient.
The ethanol gradient may be a step gradient or a linear gradient.
The virus was purified by a cesium chloride step gradient.
A step gradient can be used in order to reduce the volume of recovered polypeptide solution.
The elution can also be performed by step gradient elution.
A step gradient was used to selectively elute each lipid class from the column.
The treated lysate was pelleted through a sucrose step gradient.
DsbA is eluted from the column using a step gradient increase in salt concentration.
More preferably the elution is performed with a pH step gradient.
Consequently, a step gradient was used to elute.
Many metrics are available for characterizing differences in optical density step gradient.
The proteins on this column were then eluted with a step gradient of increasing salt concentration.
See also
The method of claim 1, wherein the pH gradient is a continuous gradient or a step gradient.
After loading, a 3 step gradient elution is used to elute the VLPs from the HIC resin.
The minimisation of the deviation E is carried out using an adaptive step gradient descent algorithm.
Step gradient for 5 min.
In further embodiments, the salt gradient is a step gradient.
Elution was performed with a step gradient of Buffer C with 1M NaCl.
Following removal of TFA, peptides can be eluted with a step gradient.
The product was eluted by a step gradient consisting of ethylacetate, dichloromethane and dichloromethane / methanol 3:1.
The EtOAc-soluble fraction was chromatographed over silica gel using a step gradient of EtOAc-MeOH as eluent.
The eluent was a step gradient of 25mM aqueous phosphate buffer ( pH7 ) and methanol.
Elution of G-CSF is performed by applying a step gradient of MilliQ water.
The column was run with a step gradient at 2.75 mL / min and fractions were collected every 8 min.
The concentrated recombinant tau protein was then eluted from the column with a 1 M NaCl step gradient.
The compound was purified by flash chromatography ( silica / step gradient from chloroform to ethyl acetate ).
Step gradient for 5 min. with C.
Figure 5 is a high temperature RP HPLC profile of rhPDGF-BB using a step gradient elution.
The modified GCSF Mutein was eluted with a step gradient using 0.15M NaCl in the equilibration buffer.
Fig . 6 shows a sensitometer strip, which can be used as an optical density step gradient.
The column was then eluted with a step gradient of 5 % ethyl acetate / hexanes through 30 % ethyl acetate / hexanes.
The interferon is run on the column preferably using a step gradient at 175mM NaCl.
The adsorbed material was eluted in a step gradient using 100 % buffer B.
PBMC were isolated with ISM ( ficol ) ( ICN Biomedicals ) by step gradient separation.
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Examples of using Gradient
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Estuaries are defined by a gradient of fresh to salt water
Gradient values and their precise location
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