Examples of 'stringency hybridization' in a sentence
Meaning of "stringency hybridization"
stringency hybridization - This is a scientific term used in molecular biology to describe the process of combining nucleic acids under precise conditions to form stable hybrids
How to use "stringency hybridization" in a sentence
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stringency hybridization
Moderate and high stringency hybridization conditions are well known in the art.
Such primers and probes hybridize specifically to a target sequence under high stringency hybridization conditions.
These high stringency hybridization conditions may be adapted by a person skilled in the art.
Such probes and primers hybridize specifically to a target molecule under high stringency hybridization conditions.
Low stringency hybridization experiments revealed the existence of hap related genes in the human genome.
An example procedure would be a combination of PCR and low stringency hybridization.
High stringency hybridization conditions are those which only allow hybridization of substantially complementary nucleic acids.
A non-limiting example of low stringency hybridization conditions is as follows.
High stringency hybridization conditions as for instance described in Sambrook et al.
A non-limiting example of high stringency hybridization conditions is as follows.
Opsin-encoding nucleic acids can be obtained under conditions of high stringency hybridization.
Preferably, low stringency hybridization conditions are used, at least at first.
In the present invention, it is preferred to use high stringency hybridization and wash conditions.
In a specific embodiment, stringency hybridization conditions are used to identify a homologous TACI protein gene.
Thus, additional LAP sequences may be readily identified using low stringency hybridization.
See also
Plasmid pGB25 was also obtained by low stringency hybridization using the pRATDPD insert as a probe.
High stringency hybridization conditions were the same except that 50 % formamide was used with 24 h hybridization.
The method of claim 28, wherein said high stringency hybridization conditions are provided.
The low stringency hybridization series was washed at room temperature in 2X SSPE containing 0.1 % SDS.
A total of 152 hybridizing clones were detected under low stringency hybridization and washing conditions.
In one embodiment, low stringency hybridization conditions are used to identify a homologous AIF peptide.
For example, 68°C are appropriate for high stringency hybridization.
An exemplary set of low stringency hybridization conditions is 50°C and 10 x SSC.
Thus, additional p28 Bap31 may be identified using low stringency hybridization.
An exemplary set of intermediate stringency hybridization conditions is 55°C and 1 x SSC.
An example of an intermediate stringency hybridization condition is 55°C and 1X SSC.
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Examples of using Stringency
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The low stringency analysis is too circumstantial
All are subject to a verification regime of unprecedented stringency
Upon the stringency of the reduction target
Examples of using Hybridization
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There was no evidence of hybridization in the wild population
The hybridization is carried out in closed loop
Method and machine for hybridization by refusion
