Examples of 'template nucleic' in a sentence
Meaning of "template nucleic"
template nucleic: Refers to a standardized or pre-designed structure for nucleic acids, such as DNA or RNA, used for various molecular biology techniques
How to use "template nucleic" in a sentence
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template nucleic
Subsequently the desulfonated template nucleic acid is amplified.
Template nucleic acids can be derived from essentially any source.
Providing a template nucleic acid.
The first step is preparing a library population of template nucleic acids.
Preferably the template nucleic acid comprises a target nucleic acid.
Such a sample could contain billions of template nucleic acid molecules.
The template nucleic acid may be encapsulated with a bead and amplified.
Method for determining the amount of template nucleic acid present in a sample.
The template nucleic acid is any convenient nucleic acid for analysis.
The fourth step is sequencing the plurality of desired template nucleic acids.
These tagged template nucleic acid molecules are copied and the copies fragmented.
Higher temperatures may promote the separation of duplex template nucleic acids.
Amplification of the template nucleic acid may comprise use of one or more polymerases.
The sequence reaction also allows a determination of the template nucleic acid length.
A template nucleic acid of this form would function as a second template in the amplification process.
See also
The primer and extension product thereof may be complementary to a template nucleic acid strand.
Template nucleic acids can also be chemically synthesized using techniques known in the art.
This allows consensus sequences for each molecule of original template nucleic acids to be determined.
A template nucleic acid of this form would function as a first template in the amplification process.
The third step is isolating a plurality of desired template nucleic acids from the mixture.
A template nucleic acid may be prepared using a nucleic acid obtained from a subject as a template.
A clonal population can be derived from a single target nucleic acid or template nucleic acid.
The primers are incubated with template nucleic acid under conditions that permit selective hybridization.
The invention provides for oligonucleotide primers for amplifying a template nucleic acid sequence.
In this embodiment the tagged template nucleic acid molecules provided will further comprise a stub sequence.
There will be multiple fragments produced corresponding to the same original template nucleic acid molecule.
The more abundant the template nucleic acid sequence the earlier the Ct is reached.
Essentially a pair of primers that flank the target sequence are incubated with a template nucleic acid.
In some embodiments, the template nucleic acid is from a biological sample.
In some embodiments, the reaction mixtures comprise a template nucleic acid.
In some embodiments, the template nucleic acid is from a viral or bacterial genome.
Nucleic acids may be selectively and specifically amplified from a template nucleic acid contained in a sample.
Fragmenting large template nucleic acid molecules to generate a plurality of fragmented nucleic acids ;.
The second step includes isolating the desired template nucleic acid from the mixture.
Optionally, the template nucleic acid or the primer nucleic acid is attached to a solid support.
Short primers generally form sufficiently stable hybrids with template nucleic acids at cooler temperatures.
The terms template, nucleic acid template, template nucleic acid and template polynucleotide are used interchangeably.
Preparing a library population of template nucleic acids ;.
A method of selecting a desired template nucleic acid from a population of template nucleic acids, comprising,.
Figure 5 depicts examples of suitable double stranded template nucleic acids.
In some embodiments, the template nucleic acid may be from a synthetic source.
Second, a plurality of sequencing primers are hybridized to the template nucleic acid or acids.
If desired, detecting a second template nucleic acid can be used as a confirmatory test.
In some embodiments, the reaction mixture further comprises a template nucleic acid.
Isolating a plurality of desired template nucleic acids from the mixture ; and.
Optionally, the second oligonucleotide includes a sequence complementary to a portion of a template nucleic acid.
Sequencing the multiple fragmented template nucleic acid molecules ; and.
The template nucleic acid is preferably DNA or RNA and may be single or double-stranded.
In fact, high concentration of the template nucleic acid favors this process.
Furthermore, the displaced downstream fragments can be extended on a secondary template nucleic acid.
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