Examples of 'template nucleic acid' in a sentence
Meaning of "template nucleic acid"
template nucleic acid: a molecule that serves as a pattern or guide for the synthesis of new nucleic acid strands
How to use "template nucleic acid" in a sentence
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template nucleic acid
Subsequently the desulfonated template nucleic acid is amplified.
The template nucleic acid may be encapsulated with a bead and amplified.
Providing a template nucleic acid.
The template nucleic acid is any convenient nucleic acid for analysis.
Such a sample could contain billions of template nucleic acid molecules.
Preferably the template nucleic acid comprises a target nucleic acid.
The sequence reaction also allows a determination of the template nucleic acid length.
These tagged template nucleic acid molecules are copied and the copies fragmented.
Method for determining the amount of template nucleic acid present in a sample.
A template nucleic acid of this form would function as a second template in the amplification process.
The primer and extension product thereof may be complementary to a template nucleic acid strand.
Amplification of the template nucleic acid may comprise use of one or more polymerases.
A clonal population can be derived from a single target nucleic acid or template nucleic acid.
A template nucleic acid of this form would function as a first template in the amplification process.
The invention provides for oligonucleotide primers for amplifying a template nucleic acid sequence.
See also
A template nucleic acid may be prepared using a nucleic acid obtained from a subject as a template.
The second step includes isolating the desired template nucleic acid from the mixture.
Fragmenting large template nucleic acid molecules to generate a plurality of fragmented nucleic acids ;.
There will be multiple fragments produced corresponding to the same original template nucleic acid molecule.
The primers are incubated with template nucleic acid under conditions that permit selective hybridization.
Essentially a pair of primers that flank the target sequence are incubated with a template nucleic acid.
In this embodiment the tagged template nucleic acid molecules provided will further comprise a stub sequence.
Nucleic acids may be selectively and specifically amplified from a template nucleic acid contained in a sample.
The more abundant the template nucleic acid sequence the earlier the Ct is reached.
In some embodiments, the reaction mixtures comprise a template nucleic acid.
In some embodiments, the template nucleic acid is from a biological sample.
Second, a plurality of sequencing primers are hybridized to the template nucleic acid or acids.
In some embodiments, the template nucleic acid is from a viral or bacterial genome.
In some embodiments, the reaction mixture further comprises a template nucleic acid.
In some embodiments, the template nucleic acid may be from a synthetic source.
Optionally, the second oligonucleotide includes a sequence complementary to a portion of a template nucleic acid.
Sequencing the multiple fragmented template nucleic acid molecules ; and.
Optionally, the template nucleic acid or the primer nucleic acid is attached to a solid support.
Constructs can be used, for example, as control template nucleic acid molecules.
A method of selecting a desired template nucleic acid from a population of template nucleic acids, comprising,.
Furthermore, the displaced downstream fragments can be extended on a secondary template nucleic acid.
In fact, high concentration of the template nucleic acid favors this process.
Optionally, the first oligonucleotide has a sequence complementary to the template nucleic acid.
If desired, detecting a second template nucleic acid can be used as a confirmatory test.
Constructs of the invention can be used, for example, as control template nucleic acid molecules.
In highly preferred embodiments, the template nucleic acid within the compartment encodes the replicase.
A method according to any preceding claim, wherein the template nucleic acid is DNA.
The terms template, nucleic acid template, template nucleic acid and template polynucleotide are used interchangeably.
The reporter oligonucleotide, upstream fragment or template nucleic acid may be labelled.
The template nucleic acid is preferably DNA or RNA and may be single or double-stranded.
In certain embodiments, the reaction mixture contains a template nucleic acid that is isolated from blood.
Providing a template nucleic acid comprising the sequence of claim 1 ; and.
A method for generating sequences of at least one individual target template nucleic acid molecule comprising,.
The template nucleic acid ( the sequence to be transcribed ) need not be double-stranded.
In some embodiments, the template nucleic acid is RNA.
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