Examples of 'two-hybrid' in a sentence
Meaning of "two-hybrid"
two-hybrid (adjective) - Refers to a genetic method used in molecular biology to study protein-protein interactions in living cells
How to use "two-hybrid" in a sentence
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two-hybrid
Yeast two-hybrid systems are also applicable.
Screening of cDNA by yeast two-hybrid system.
In the two-hybrid system, interacting proteins are fused to domains of transcription factors.
Principles of yeast and mammalian two-hybrid systems.
Two-hybrid systems may also be used to detect protein interactions in vivo.
Eight independent clones isolated in this two-hybrid screen were characterized.
Two-hybrid screens can provide an important first hint for the identification of interaction partners.
These interactions will be explored by using the yeast two-hybrid system.
Such methods include the yeast two-hybrid screening that is described in example 1.
In vivo assays may also take the form of two-hybrid assays.
In bacterial two-hybrid system, some interactions happen with protein implicated in development and stress.
A plasmid comprising the mammalian reverse two-hybrid system according to claim 1.
The yeast two-hybrid assay is useful to identify proteins that bind to the gene product.
A mammalian cell comprising a mammalian reverse two-hybrid system according to claim 1.
The two-hybrid system is a genetic assay for detecting interactions between two proteins or polypeptides.
See also
Afterwards, the recombined zinc fingers are screened vía a bacterial two-hybrid system.
Such methods include yeast and mammalian two-hybrid assays and co-immunoprecipitation techniques.
Binding or interaction of two components may also be determined using a two-hybrid assay.
These compounds are detected by adapting yeast two-hybrid expression systems known in the art.
For example, an interaction can be determined by functional assays such as the two-hybrid systems.
The two-hybrid system involves two plasmid vectors each intended to encode a fusión protein.
Many of them have been developed specifically for certain yeast two-hybrid systems.
The yeast two-hybrid system is a commonly used experimental technique for the study of binary interactions.
One preferred approach using yeast takes advantage of yeast two-hybrid techniques.
Although the yeast two-hybrid system has proven highly useful, it suffers from a number of limitations.
One method that detects protein interactions in vivo is the two-hybrid system.
The yeast two-hybrid system is a genetic method that overcomes certain shortcomings of the above approaches.
These same interactions can be detected using the yeast two-hybrid system.
A two-hybrid selection system may also be applied making appropriate constructs with the available chemokine sequences.
Binding is measures by methods known in the art such as a yeast two-hybrid screening system.
The two-hybrid assay is generally used for identifying proteins that interact with a particular target protein.
Novel protein-protein interactions have been discovered and confirmed using yeast two-hybrid systems.
This vector was used as bait in a two-hybrid screen of a human B cell cDNA library.
Alternatively, the nucleic acid molecules of the invention can be used in a yeast two-hybrid system.
Results of the he Yeast Two-Hybrid Screening clones were obtained, sequenced and analyzed.
The present invention also comprises plasmids comprising a mammalian reverse two-hybrid system of the invention.
For example, the two-hybrid assay system described in the Examples can be adapted accordingly.
Our research group has previously identified the interaction between ankhd1 and siva through two-hybrid assay.
Libraries used in the two-hybrid system have clones expressing GAL4 activation domain fusión proteins.
Is a diagrammatic illustration of the DD interactions observed in the yeast two-hybrid tests.
A two-hybrid selection system may also be applied making appropriate constructs with the available Langerin sequences.
Another suitable ligand-binding assay is the yeast two-hybrid system Fields et al.
A two-hybrid selection system may also be applied making appropriate constructs with the available DTLR sequences.
Vectors Many types of vectors can be used in a transcription-based two-hybrid assay.
PCR was performed on an Arabidopsis / yeast two-hybrid suspension culture cDNA library.
Accordingly, the inventors identified a protein which binds to CAP by a yeast two-hybrid system.
Using a yeast two-hybrid screen we identified several potential interactingpartners for TRKC KF.
In principle, the interaction of MIF with other proteins can be tested with a two-hybrid system.
We also use Two-Hybrid data in our study.
LexA has been used to provide the DNA binding function in the yeast two-hybrid system.
