Examples of 'two-hybrid system' in a sentence
Meaning of "two-hybrid system"
two-hybrid system: A scientific method or approach that involves the interaction of two components or entities, often used in biological studies or experiments
How to use "two-hybrid system" in a sentence
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two-hybrid system
Screening of cDNA by yeast two-hybrid system.
The two-hybrid system is a genetic assay for detecting interactions between two proteins or polypeptides.
These interactions will be explored by using the yeast two-hybrid system.
The two-hybrid system involves two plasmid vectors each intended to encode a fusión protein.
A plasmid comprising the mammalian reverse two-hybrid system according to claim 1.
The yeast two-hybrid system is a commonly used experimental technique for the study of binary interactions.
A mammalian cell comprising a mammalian reverse two-hybrid system according to claim 1.
Although the yeast two-hybrid system has proven highly useful, it suffers from a number of limitations.
Afterwards, the recombined zinc fingers are screened vía a bacterial two-hybrid system.
The yeast two-hybrid system is a genetic method that overcomes certain shortcomings of the above approaches.
One method that detects protein interactions in vivo is the two-hybrid system.
Libraries used in the two-hybrid system have clones expressing GAL4 activation domain fusión proteins.
These same interactions can be detected using the yeast two-hybrid system.
Furthermore, we are employing, a yeast two-hybrid system to detect interacting proteins.
Alternatively, the nucleic acid molecules of the invention can be used in a yeast two-hybrid system.
See also
A mammalian reverse two-hybrid system for identifying a change in protein-protein interaction comprising,.
The present invention also comprises plasmids comprising a mammalian reverse two-hybrid system of the invention.
A unified yeast and bacterial two-hybrid system for practicing the method of claim 7, comprising,.
Another suitable ligand-binding assay is the yeast two-hybrid system Fields et al.
Alternatively, the yeast two-hybrid system may be a tool for the identification of protein-protein interactions.
Accordingly, the inventors identified a protein which binds to CAP by a yeast two-hybrid system.
Features of the yeast two-hybrid system ( adapted from Clontech manual ).
In principle, the interaction of MIF with other proteins can be tested with a two-hybrid system.
The two-Hybrid system used for screening was the Matchmaker version III ( Clontech ).
LexA has been used to provide the DNA binding function in the yeast two-hybrid system.
The two-hybrid system.
Interaction of Bbk point mutants with Bak analyzed by yeast two-hybrid system.
The mammalian reverse two-hybrid system according to claim 15, wherein said kinase is a disease-related kinase.
Preferably, the method of the invention is based on a two-hybrid system.
In the yeast two-hybrid system the carboxyl terminus of MyD116 interacted with the human PP1a.
Such polypeptides can be found, for example, using the yeast two-hybrid system.
We used the SRS yeast two-hybrid system from Stratagene ( CytoTrap ) to identify Hsp27 protein interactors.
Figures 4A and 4B depict the results of screening of interacting proteins with the bacterial two-hybrid system.
For example, the yeast two-hybrid system developed at SUNY described in U.S. Patent No.
To overcome this problem a C. albicans specific two-hybrid system was developed.
Figure 3A illustrates the interaction between THAP1 and PAR4 in a yeast two-hybrid system.
Vectors and strains used were provided with the Matchmaker Two-Hybrid System ( Clontech, Palo Alto, CA ).
These problems can potentially be overcome with an E. coli two-hybrid system.
Example 1 Detection of proteins interacting with NIK by the two-hybrid system method,.
Self-interaction of GPBP and GPBPA26 assessed by a yeast two-hybrid system.
Figure 4 . Specific interaction between NAC1 and NAC1 in the yeast two-hybrid system assay.
Figure 3, Diagram outlining the mechanism of this E. coli two-hybrid system.
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The system is shipped as two separate components
Examples of using Two-hybrid
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Yeast two-hybrid systems are also applicable
Screening of cDNA by yeast two-hybrid system
In the two-hybrid system , interacting proteins are fused to domains of transcription factors
