Examples of 'using primers' in a sentence
Meaning of "using primers"
Using primers typically refers to the process of applying a preparatory coating or substance, known as a primer, onto a surface before painting, coating, or applying another layer. Primers are used to enhance adhesion, improve durability, provide corrosion resistance, or create a suitable surface for further treatment, ensuring better results and performance
How to use "using primers" in a sentence
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using primers
A modification was made by using primers bearing a fluorophore.
In embodiments in which the donor polynucleotide is isolated by PCR using primers FIG.
Null cells were genotyped using primers specific to both insertional alleles.
Amplification reactions are conducted on the pooled BAC clones using primers specific for the STSs.
The cDNA is then amplified using primers to conserved regions of human immunoglobulins.
Normalization was performed by PCR using primers to acfin.
This is a PCR using primers which specifically recognize the elite event.
A nucleotide sequence which can be amplified using primers disclosed herein.
Probes were generated using primers described below in a PCR reaction under the following conditions.
Also provided is a method for the amplification using primers specific for bacterial species.
PCR screening using primers detecting the gene lpo8 was performed.
Intron sequence can be isolated from natural sources using primers based on known sequence.
For example, PCR using primers incorporating fluorescent probes is one suitable technique.
Normalization was performed by PCR using primers to actin.
RT-PCR was performed using primers that flank the td site of insertion.
See also
Genomic DNA from those ten strains are then analysed using primers identified as SEQ.
This was done using primers shown in Table 8.
Similarly a soluble version of human LRP5 can be constructed using primers shown in Table 9.
Sequence analysis was performed using primers that flank the scFv of the display vector.
PCR using primers specific to the predicted coding sequence of zcyto22 was also carried out.
The initial sequence was obtained using primers that annealed to the cloning vector.
Excellent storage stability ; and Good adhesion properties without the necessity of using primers.
The antibody gene is therefore amplified using primers which contain the two restriction sites.
The GAPDH promoter of P. pastoris was amplified using primers.
A second PCR reaction is performed using primers designed to amplify the region of insertion.
Mixing the products obtained in ( iii ) above and performing a PCR reaction using primers A and D.
The genes of interest are PCR amplified using primers in which one is fluorescently labelled.
An overlap between primers 2 and 3 allowed to connect both fragments vía PCR using primers 3 and 4.
The quality of the DNA was confirmed using primers specific for a control gene.
Using primers specific for the pmi gene, a 788 bp fragment was amplified using PCR.
Amplification was performed on each DNA sample using primers designed as previously explained.
Expression studies using primers for CCR6 revealed a high variability of CCR6 mRNA expression.
DNA sequencing was performed by gene walking using primers designed for the lbpB genes.
The basis for using primers C / D is different from that of other two sets.
Normalization was performed by PCR using primers to actin and GAPDH.
Amplification using primers P1 and cP2 yields amplicons that can be sequenced.
Normalization was performed t PCR using primers to actin and GAPDH.
A PCR reaction was performed using Primers A and DVW applied to the single-stranded sources listed above.
The hygromycin gene cassette is PCR amplified using primers identified as SEQ.
PCR was performed using primers which amplify 306 bp of the caa gene.
Agarose gel of PCR products generated using primers SML and SMR.
Sequencing was performed using primers and PCR cycling conditions as described by Samuels et al [ 1 ].
In some embodiments, amplification can be performed using primers that are gene specific.
Quantitation was obtained using primers and a Taqman ™ fluorescent probe derived from the ADAM8-encoding gene.
Figure 2 shows the results of allele-specific amplification using primers with internal base modifications.
Figure 39 PCR results using primers with 5 ' overhangs containing Solexa adapter sequences.
Amplification of specific genes was done using primers showed in Table 1.
Quantitation was obtained using primers and a TaqMan ™ fluorescent probe derived from the TASK110-encoding gene.
Normalization can be performed by PCR using primers to actin and GAPDH.
PCR was then performed using primers specific for the pCMV-s plasmid.
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