Examples of 'xho' in a sentence
Meaning of "xho"
Xho is a term that is not commonly used in English. It may refer to a specific language or culture
How to use "xho" in a sentence
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xho
This fragment is cloned into the Xho I site in a plasmid vector.
The transfer of the dengue cDNA fragment made use of this Xho I site.
Plasmids were analyzed by Xho I digestion and agarose gel electrophoresis.
This fragment was flanked by the restriction sites Xho i and Hind III.
The Xho I cut ends were then filled in and ligated back.
The correct orientation was tested for by Xho I digestion.
It contains a unique Xho I restriction enzyme site into which foreign DNA may be inserted.
The amplified fragments were digested with Xho I and gel purified.
The Xho I recognition site was lost as a result of the insertion, as shown below,.
The cDNA is then digested with the Xho I restriction enzyme.
Two opposite orientations of the insert were distinguished by cutting with Hind III and Xho I.
The chromosomal DNA was first digested with Xho I to give shorter templates.
The Xho I restriction site at the 3 ' end of the cDNA had been previously introduced.
The ends are filled in with Klenow polymerase and Xho I linkers added.
The ligation product was digested with Xho 1 prior to transformation to eliminate parental plasmid.
See also
However, this site can be cleaved with Xho II.
This oligonucleotide also introduced a Xho I site just downstream of the IL-3 stopcodon.
Briefly, the first cDNA was constructed using an oligo dT primer with Xho I adapters.
Nco I / Xho I digests were performed to determine which clones had the correct size of fragments.
This conclusion was confirmed by results obtained with the methylation-sensitive restriction enzyme Xho I.
The ligation products are linearized with Xho I to allow run-off transcription.
The Xho I restriction site for cloning into the pComb2-3 vector is underlined.
The ends were filled-in with Klenow polymerase and Xho I linkers were added.
Linearising the plasmid with Xho I and transcribing with SP6 generated the antisense probe.
The 5 ' end of the sense primer contains two consecutive Xho I restriction sites.
The first primer contains an Xho I site and the second an Nhe I site ( boldface letters ).
Future constructs are being created wherein the base downstream of the Xho 1 site is a " C " nucleotide.
The cDNA was then digested with Xho 1 restriction enzyme ( Stratagene ) using standard molecular biology procedures.
The 3 ' oligonucleotide contained an Xho I restriction site.
As shown, NIa IV / Xho I fragment has an enhancer activity.
The 5 ' end of the primer contains a Xho I restriction site.
Shown also is the Xho I site ( X ) where applicants have made their insertion.
CATXY2 was used as the reverse primer and contains the Xho I site ( CTCGAG ) for cloning.
Subsequently a 6 bp Xho I linker is ligated into the Hind III site resulting in pBD10.
The oligonucleotide designed for the 3 ' end incorporates an Xho I site followed by 20 untranslated nucleotides.
The resulting gpt expression construct is designated pBGS 131 di Xho I-gpt.
The PCR products were inserted into the EocRI and Xho I sites of pCAGGSn3FH-HA expression vector.
Xho I cleavage of this vector deletes a 102 bp segment of VEE nsP3.
This primer contains an Xho I site at the 5 ' end of the primer.
DNA samples were adjusted to 0.2mg/ml and digested with the restriction enzyme Xho I.
This vector was digested with Xho I and PmeI simultaneously overnight at 37°C.
Set 1 was digested with Dra I and probed with the Pst l - Xho l fragment of pUPC30.
The insert included a new Xho I site at the 3 ' end of the HA sequence.
Nco I and Xho I restriction sites were introduced at the 5 ' and 3 ' ends.
Insertion of this oligonucleotide resulted in a Xho I site-deleted plasmid, designated pBGS 131 dl XhoI.
The 5 ' Hind III and 3 ' Xho I sites were added to assist subcloning into a vector.
The fragment was gel-purified, cut with Xho I and gel-purified.
The cDNA was linearized with Xho I to facilitate in vitro transcription by SP6 polymerase ( Epicentre ).
We digested this plasmid with Aat II and Xho I and isolated the 4391 bp fragment.
Plasmid pBTL24 was digested with Xho I and Hind III to isolate the 2.4 kb expression unit.
