Examples of 'xhoi' in a sentence
Meaning of "xhoi"
XHOI (noun): XhoI is a type of restriction enzyme used in molecular biology to cut DNA at specific recognition sites. It is commonly used in genetic engineering techniques such as DNA cloning and gene mapping
How to use "xhoi" in a sentence
Basic
Advanced
xhoi
The gene contained no XhoI site.
After digestion with XhoI the molecule is recircularised by ligation.
The pooled samples are digested with XhoI and NotI.
A XhoI and a BglII sites were introduced with the primers.
Typical cloning sites that were used are XhoI and NotI.
This unique XhoI cloning site is at the junction of the upstream and downstream sequences.
The termini were prepared by digestion with PstI and XhoI.
This plasmid was cut with XhoI and partially with BamHI.
This fragment was isolated and digested with XhoI and NcoI.
Restriction sites KpnI and XhoI served as insertion sites for heterelogous genes to be expressed.
The resulting plasmid was then digested with HindIII and XhoI.
The fragment is cut with PstI and XhoI and purified from an agarose gel.
The restriction sites used in the subcloning are EcoRI and XhoI.
The resulting plasmid contains an XhoI site upstream of the heavy chain translation initiation codon.
The other experimental conditions are the same as those described for XhoI.
See also
The XhoI site will be regenerated and the BgIII and BamHI sites will be eliminated.
The amplified fragment was then excised by digestion with NdeI and XhoI.
XhoI and SalI restriction sites are framed.
The resulting intermediate construct is then digested with AscI and XhoI.
XhoI linkers are added as described above.
Immediately downstream of this is the ElonginC gene cloned into the XhoI site to SacII.
The positions of the XhoI linker were determined by DNA sequencing.
The amplimers were ligated to the linear pczHFVenv wt plasmid using the XhoI redundant ends.
The XhoI end was made blunt by use of the Klenow fragment.
The latter is digested with the restriction enzymes sphI and xhoI on the lacZ gene.
The position of the XhoI site used for insertion of a or B subunit sequences is shown.
The amplified fragment was then excised by cleavage with the two enzymes NdeI and XhoI.
The restriction sites EagI and XhoI which flank the promoter originate from the PCR primers.
Those transformants carrying pADHt will have regenerated BamHI and XhoI sites in their plasmids.
The plasmid obtained which has an XhoI linker inserted thereinto was termed pE12ΩGUSx.
These were also subcloned into the modified pCI vector at the XhoI and BamHI sites.
In addition this fragment contains a XhoI and a HindIII restriction site at its 3 ' end.
The termini were prepared by digestion with Nhel and XhoI.
These primers provide, in addition, the SacI and XhoI enzymatic restriction sites respectively.
The PCR product was digested with BsiWI and XhoI.
The cloning vector pSW1 was digested with XhoI and XbaI to yield two fragments.
The DNA is ethanol precipitated and further digested with XhoI.
The restriction enzymes XhoI and SalI have compatible ends, which enabled proper ligation.
The fragment produced by the PCR was hydrolysed by XhoI and XcmI.
Next the XhoI site located outside of the T-DNA borders was deleted.
This fragment was treated with Klenow fragment before addition of XhoI linkers.
PBlueII-HI was digested with XhoI and NotI to excise the hybrid intron fragment.
The PCR product is digested with the restriction enzymes xhoI and speI.
Oligonucleotide 4, Designed to insert a XhoI site just after the stop codon of pepE.
Both ends of the DNA were cut by use of restriction enzymes NdeI and XhoI.
The DNA is digested with XhoI and BamHI.
The PCR product thus obtained is digested with the restriction enzymes apaI and xhoI.
Overlap between cosmids was initially determined by XbaI + XhoI digestion and Southern blotting.
The purified PCR product was restricted in a double digest using the enzymes NdeI and XhoI.
A XhoI - XbaI fragment containing the amino terminus of the Factor VII sequence was isolated.
