Examples of 'exonuclease' in a sentence
Meaning of "exonuclease"
Exonuclease (noun): A type of enzyme that cleaves nucleotides one at a time from an end of a polynucleotide chain, playing a crucial role in DNA replication and repair processes
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- Any of a group of enzymes which cleave single nucleotides from the end of a polynucleotide (DNA or RNA) chain.
How to use "exonuclease" in a sentence
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exonuclease
It is disclosed the exonuclease is a plant DEDDh exonuclease.
The first oligonucleotide can also be degraded by an exonuclease.
In other embodiments the exonuclease is not lambda exonuclease.
These modified oligonucleotides are reported to be resistant to exonuclease activity.
Enzymes having exonuclease activity are known in the art.
Fluorescence increases when hybridization or exonuclease hydrolysis occurs.
Lambda exonuclease comes from.
Overlapping fragments are trimmed with an exonuclease.
This traps the exonuclease into a solid state.
The resulting mRNA fragments are then destroyed by exonuclease.
It does not have the exonuclease activity.
Each type of exonuclease has a specific type of function or requirement.
Unligated products are eliminated by exonuclease digestion.
The action of λ exonuclease occurs on the hybridised probe sequence.
Any overlapping unhybridized fragment ends are trimmed down by an exonuclease.
See also
How is the action of exonuclease different.
An exonuclease degrades from the ends of a nucleic acid molecule.
The ends are then removed by exonuclease to expose the repeats.
Exonuclease activity assays.
This synthesis and exonuclease phases may be repeated in cycles.
The oligonucleotides of the invention are particularly stable to exonuclease degradation.
The addition of an exonuclease avoids unwanted side products during amplification.
It is important that the enzyme used lack exonuclease activity.
This method exploits the exonuclease activity of a polymerase to generate a signal.
The degradation enzyme is an exonuclease.
One probe design depends on exonuclease hydrolysis for signal generation.
This may be prevented by choosing long fragments after exonuclease treatment.
Such exonuclease activity is not detrimental to the amplification methods of the invention.
Heat inactivation of exonuclease digestions.
This investigated the effect of doubling the concentration of lambda exonuclease.
The ratio between polymerase and exonuclease has to be optimized.
Heterologous cleavage domains can be obtained from any endonuclease or exonuclease.
It is free of detectable exonuclease activity.
The products of the amplification are subsequently treated with a lambda exonuclease.
Also disclosed are compositions comprising an exonuclease of use in the invention in a buffer.
Apparently this exonuclease plays an important role in the metabolism of the bacteria.
Some nucleases have both endonuclease and exonuclease activities.
The digestion with lambda exonuclease was performed as described in the experimental section.
The best mutants are chosen for further exonuclease activity.
The preferred exonuclease cleavage site is a phosphodiester bond in the double helical region.
Such modified linkages are known to be resistant to exonuclease and endonuclease activity.
The signal generated by exonuclease probes is cumulative and only indirectly related to product concentration.
The amplification product is contacted with an exonuclease to generate deletions therein.
Methods of the present invention may further comprise the addition of an exonuclease.
It is therefore impossible to use an exonuclease step to remove unligated side products.
Arrows indicate the cleavage products resulting from exonuclease cleavage.
The admixture preferably includes an exonuclease and a glycosylase and at least one antibody.
Appendages to the ends of the oligonucleotide chain also provide exonuclease resistance.
The altered polymerase can comprise reduced exonuclease activity as compared to a wild type polymerase.
Appendages to the ends of the oligonucleotide chains also provide exonuclease resistance.
