Examples of 'exonucleases' in a sentence
Meaning of "exonucleases"
Exonucleases (noun): Enzymes that cleave nucleotides one at a time from the end of a polynucleotide chain
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- plural of exonuclease
How to use "exonucleases" in a sentence
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exonucleases
Such nucleases include endonucleases and exonucleases.
Suitable exonucleases will be evident to the skilled worker.
Such enzymes include polymerases and exonucleases.
Use of exonucleases to destroy unligated probes and target.
A similarly good fit is yet to be demonstrated with exonucleases.
Exonucleases and polymerases can be advantageously used for this purpose.
Selective RNAses may be endonucleases or exonucleases.
Exonucleases and glycosylases can also be included in the reaction mixture.
Fragmentation is then amplified with exonucleases.
Exonucleases may cleave the phosphodiester bonds at blunt ends or sticky ends.
The molecule is slowly broken up by endonucleases and subsequently by exonucleases.
Such exonucleases are commercially available or can be obtained by methods known in the art.
Unmodified oligonucleotides or polynucleotides are cleaved in vivo by endonucleases and exonucleases.
In further embodiments the exonucleases of the invention have substantially no endonuclease activity.
It was established that these difficulties were due to the presence of exonucleases.
See also
The use of exonucleases for nucleic acids and exopeptidases for proteins allow partial digestion.
Endonucleases are generally preferred as more efficient liquification agents than exonucleases.
Experiments were therefore performed to test different exonucleases for the fragmentation of ssDNA.
Exonucleases are enzymes which cleave nucleotide molecules from the ends of the nucleic acid molecule.
Biological fluids typically contain a mixture of both endonucleases and exonucleases.
The polyadenylation sequence may protect the mRNA from exonucleases thereby stabilizing mRNA production.
Exonucleases are enzymes that nonspecifically trim RNA from both ends.
Still further modified forms include dimers or trimers of the exonucleases of the invention.
Exonucleases include exonuclease III and exonuclease VII.
LNAs are resistant to exonucleases and heat.
Nucleic acid Molecular Beacons are susceptible to degradation by endonucleases and exonucleases.
In general, exonucleases are involved in a multitude of different cellular processes.
Pegaptanib is metabolised by endo - and exonucleases.
In contrast, exonucleases cleave nucleic acid chains from the ends.
Examples of enzymatic decellularizing agents are proteases, endonucleases, and exonucleases.
The exonucleases of use in the invention may be provided in a modified form, e . g.
For example, such bases may be subject to cleavage by exonucleases.
The exonucleases of the invention may therefore be recombinant enzymes, in particular isolated recombinant enzymes.
Antisense oligonucleotides are sensitive to nuclease degradation, and mainly to the action of exonucleases.
Exonucleases may also cause shortening of this junction, however this process is still poorly understood.
In another embodiment, stability of the mRNA is increased by circularization, decreasing degradation by exonucleases.
Exonucleases that preferentially attack double-stranded DNA are particularly preferred.
In other embodiments, a single polynucleotide encodes the endonucleases and exonucleases separated by IRESs.
Exonucleases and / or endonucleases, and is therefore stable in vivo.
These results suggest that pseudo-cyclic structure formation provides antisense oligonucleotides with stability against exonucleases.
Exonucleases and / or endonucleases, and are therefore stable in vivo.
PURE extracts can also be made, eliminating the concern of exonucleases.
The exonucleases of the invention may be provided in a modified form, e . g.
In another embodiment, stability of RNA is increased by circularization, decreasing degradation by exonucleases.
The term “ nuclease ” is intended to include exonucleases and endonucleases.
In another example, stability of RNA is increased by circularization, decreasing degradation by exonucleases.
Examples of exonucleases are,.
Thus, the whole DNA construct is protected against degradation by exonucleases.
Where appropriate, the exonucleases of the invention may be isolated from a natural source, e . g.
This provides significant resistance to 5 ′ exonucleases.
