Examples of 'exonuclease activity' in a sentence
Meaning of "exonuclease activity"
exonuclease activity - This term is used in molecular biology to describe the capability of an enzyme to degrade nucleic acids by removing nucleotides one by one from the end of a chain.
How to use "exonuclease activity" in a sentence
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exonuclease activity
Enzymes having exonuclease activity are known in the art.
These modified oligonucleotides are reported to be resistant to exonuclease activity.
This method exploits the exonuclease activity of a polymerase to generate a signal.
It is important that the enzyme used lack exonuclease activity.
Exonuclease activity assays.
It does not have the exonuclease activity.
Such exonuclease activity is not detrimental to the amplification methods of the invention.
It is free of detectable exonuclease activity.
The 5 ' exonuclease activity of DNAP Taq was reported to require concurrent synthesis Gelfand.
The best mutants are chosen for further exonuclease activity.
To quantify this, we tested klenow exonuclease activity using the assay described above in example 3.
Analogous methods may be applied for identifying suitable conditions for other enzymes having exonuclease activity.
The reaction products were analyzed by homochromatography and exonuclease activity was quantified following autoradiography.
The primer extension reaction is performed with a polymerase that significantly lacks 5 ' -3 ' exonuclease activity.
The altered polymerase can comprise reduced exonuclease activity as compared to a wild type polymerase.
See also
Because of its amide backbone, it can not be hydrolyzed by exonuclease activity.
Hydrolysis of the probe by polymerase exonuclease activity releases the donor dye from quenching.
The kit may also include a nucleic acid polymerase comprises a 5 ' exonuclease activity.
The time course of loss of exonuclease activity is shown in Fig.
It is preferred that a DNA polymerase does not display non-specific exonuclease activity.
Other mutations should reduce exonuclease activity further, or completely.
A method according to claim 19 wherein said DNA polymerase has no detectable exonuclease activity.
It can not serve as a substrate for exonuclease activity by Taq polymerase.
In another embodiment the amplification employs a polymerase enzyme having 5 ' to 3 ' exonuclease activity.
The method of claim 7, wherein the exonuclease activity of the said polymerase is activated.
The polymerase of claim 1 wherein said DNA polymerase has an exonuclease activity removed.
Finally, the 5 ' to 3 ' exonuclease activity of polymerases generally also contains an inherent RNase H activity.
The internal mismatch is an inefficient substrate for the exonuclease activity of a proofreading enzyme.
Exonuclease activity is halted when the enzyme encounters exonuclease resistant hybridisation region 42 ( Figure 3c ).
Further, the polymerase has no associated exonuclease activity.
Thus, the exonuclease activity can be better described as a structure-dependent single-stranded endonuclease ( SDSSE ).
As a result, ligands that are not substrates for exonuclease activity are desirable.
Thus, a 3 ' - S ' exonuclease activity is a desired characteristic of a thermostable DNA polymerase used for such purposes.
Pol α has limited processivity and lacks 3 ′ exonuclease activity for proofreading errors.
In some embodiments, the polymerase used for extension lacks or substantially lacks 5 ' exonuclease activity.
When double stranded substrate is used the exonuclease activity is about 7-fold higher.
In another embodiment the kit further comprises a polymerase enzyme having 5 ' to 3 ' exonuclease activity.
By normal or naturally associated level is meant the exonuclease activity of unmodified T7-type polymerase.
The polymerase used for the reaction has strand displacement activity and lacks 5 ' to 3 ' exonuclease activity.
The isolated T7 polymerase has exonuclease activity associated with it.
A process according to Claim 1, wherein said DNA polymerase lacks exonuclease activity.
Preferably, the DNA polymerase lacks 5 ' to 3 ' exonuclease activity and possesses strand displacement activity.
The polymerase can have a peptide tag that does not influence the 5 ′ to 3 ′ exonuclease activity.
FIG . 10 shows a test of exonuclease activity of the Lambda Exonuclease.
LNAs are widely resistant to endo - and exonuclease activity.
The 5 ' to 3 ' exonuclease activity is preferably provided by the polymerase.
Such an enzyme preferably lacks 5 ' exonuclease activity.
Pol ζ lacks 3 ' to 5 ' exonuclease activity and is a moderate fidelity polymerase.
The amplification is performed using a DNA polymerase having 5 ' to 3 ' exonuclease activity.
Although the Klenow DNA polymerase I fragment lacks 5 ' - > 3 ' exonuclease activity it contains persistent strand displacement activity.
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Examples of using Exonuclease
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It is disclosed the exonuclease is a plant DEDDh exonuclease
The first oligonucleotide can also be degraded by an exonuclease
In other embodiments the exonuclease is not lambda exonuclease
Examples of using Activity
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Activity cards divided into four themes
Refrain from physical activity until symptoms are gone
