Examples of 'ligation mix' in a sentence
Meaning of "ligation mix"
ligation mix: A combination of DNA fragments and ligase enzyme used in molecular biology to join DNA strands together. This is a crucial step in various molecular biology techniques like cloning and PCR
How to use "ligation mix" in a sentence
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ligation mix
Two uL of the ligation mix were added to the competent cells.
A lactose-inducible P. fluorescens strain was electroporated with the ligation mix.
The ligation mix was purified by phenol extraction and ethanol precipitated.
Usually only 5 µl of the ligation mix was used for transformation.
The ligation mix was then used as a template in PCR.
Coli DH5-a cells were transformed with the ligation mix and recombinants screened for hemolysin production.
The ligation mix was transformed into a useful laboratory strain of E.
The fragments were ligated and the ligation mix used to transform E. coli strain DH5a.
The ligation mix was transformed into DH5a.
These two fragments were ligated, and the ligation mix was used to transform competent E. coli.
From this ligation mix a plasmid was recovered whose DNA was not cleaved with EcoRI.
A P. fluorescens lactose-inducible strain can be electroporated with the ligation mix.
The ligation mix was used to transform competent DH5a cells.
Coli HB101 cells were transformed with ligation mix and spread on culture plates.
The ligation mix was used to transform competent TG2 cells.
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Coli HB101 cells are transformed with ligation mix and spread on culture plates.
This ligation mix was used to transform E. coli.
For irradiating, 1 µL of 100 mM DTT can be added to the annealed ligation mix.
An aliquot of the ligation mix was used to transform competent TG2 cells.
The ligation mix was transformed into E. coli and DNA was prepared from the clones obtained.
Coli JM105 was transformed with the ligation mix containing the two fragments described above.
The ligation mix was transformed into competent E. coli.
One half of the total ligation mix was used to transform competent JM103 cells.
The ligation mix was transformed into E. coli cells for plasmid propagation.
After 24 hours the ligation mix was packaged in vitro into lambda phage.
The ligation mix was cut with Not1 prior to transformation to prevent recircularisation of pWDH443.
Following assembly, the resulting ligation mix was used to transform competent E. coli strain DH5a.
The ligation mix was chemically transformed into E. coli RRIAM15 and spread on ampicillin plates.
An aliquot of the ligation mix was introduced by electroporation into E. coli strain XLB1.
Each ligation mix was used to transform AG-1 cells.
The resulting ligation mix was used to transform E. coli TG1 cells.
The ligation mix is transfected into TOP 10 cells ( Invitrogen ) and several clones are picked.
The resultant ligation mix was used to transform E. coli DH5aF ' by standard methods.
The ligation mix was used to transform competent E. coli TG2 cells.
Transformation of the ligation mix into E. coli DH1 competent cells yielded plasmid pT-CR1c7.
The ligation mix was transformed to B . subtilis DB104 and selected for neomycin resistance ( see above ).
Aliquots of the ligation mix representing 100 nanograms of DNA were then transformed into S. lividans 66.
The ligation mix was transformed into E. coli DH11S and ampicillin-resistant colonies selected.
The ligation mix was transformed into E. coli DH5a.
The ligation mix is transformed into E . coli WK6.
The ligation mix was then precipitated, and redissolved in 30µl of TE-buffer.
This ligation mix was transformed into Lactococus lactis MG1363.
The ligation mix was used to transform competent E. coli DM5-a.
The ligation mix was used to transform E. coli DH5amcr competent cells ( BRL ).
The ligation mix was used to transform frozen, competent E. coli NM522 cells.
The purified ligation mix was then transformed into TG1 electrocompetent cells ( Agilent ) using electroporation ( BIO-RAD ).
The ligation mix was used to transform E. coli TOP10 cells.
The ligation mix was electroporated into electrocompetent E . coli TOP-10 cells.
The ligation mix was transformed into the widely available E. coli strain NM522.
The ligation mix was transformed to chemically competent dam-ldcm - E. coli cells, New England Biolabs ; Cat.
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