Examples of 'ligation mixture' in a sentence
Meaning of "ligation mixture"
A term often used in the field of molecular biology to refer to a combination of DNA fragments and ligase enzyme. The ligation mixture is used to join DNA fragments together and create recombinant DNA molecules
How to use "ligation mixture" in a sentence
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ligation mixture
The ligation mixture is used to obtain a transformant.
Host cells are transformed with the ligation mixture.
The ligation mixture was used directly for transformation.
Coli cells were transformed with the ligation mixture.
The ligation mixture may further comprise a divalent cation.
Coli cells are transformed with the ligation mixture by conventional procedures.
The ligation mixture is then transformed into a suitable host.
Transformation of ligation mixture.
The ligation mixture was used directly to transform competent cells.
The composition of the ligation mixture is the following,.
The ligation mixture is digested by FseI before transformation of the bacteria.
In an alternative scenario the ligation mixture is amplified in a 2-step approach.
The ligation mixture was then used to transform chemical competent cells.
After purification, T4 ligase was added to the ligation mixture to ligate the fragments.
The resulting ligation mixture can be considered as the cDNA library.
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After transformation of the ligation in E. coli bacteria, the ligation mixture was plated out.
Then this ligation mixture was digested with AvrII and BglII.
A ribonuclease ( RNase ) inhibitor may also be added to the ligation mixture.
The ligation mixture was transformed the next day into competent DHSa cells.
Coli JM109 was transformed with this ligation mixture as previously described.
The ligation mixture was electroporated into bacteria and propagated under appropriate antibiotic selection.
Following ligation, TOP10 cells were transformed with the ligation mixture.
Addition of thiols to the ligation mixture also keeps the reaction mixture in a reduced form.
The large fragment is then mixed with synthetics 5A/B ( defined below ) in a standard ligation mixture.
This ligation mixture was then electroporated Tauch et al.
MG1614 protoplasts were transformed with this ligation mixture selecting for chloramphenicol resistance.
The ligation mixture was separated on a GTG agarose gel.
Following incubation for 2 hours, the new ligation mixture is used to transform E. coli.
The ligation mixture is then used to transform the strain RRI.
Coli DH10B was transformed with the ligation mixture.
Aliquots of the ligation mixture are then added in a transformation experiment Cohen et al.
Transformation of E. coli, TGI was performed by using 2 µl of ligation mixture.
The ligation mixture was then used to transform the E.
Competent Bacillus subtilis RS303 cells were transformed by the ligation mixture.
The ligation mixture may further comprise ATP.
Competent cells of Bacillus subtilis RS303 were transformed with a ligation mixture.
The ligation mixture was used to transform E.
Competent E. coli DH5 a cells were transformed with 5µl of the ligation mixture.
This ligation mixture was used to transform E.
Following ligation, competent E. coli TOP10 cells were transformed with the ligation mixture.
The next day the ligation mixture was transformed into competent DH5a cells.
Competent Escherichia coli XL-Blue cells ( Stratagene, USA ) were transformed with the ligation mixture.
The ligation mixture was transformed into competent TB 1 cells.
Coli JM109 was transformed by electroportation using ligation mixture and plated on LB ampicillin plates.
The ligation mixture was used to transform competent TG2 cells.
Small scale DNA preparations were made from 36 E. coli colonies transformed with this ligation mixture.
An aliquot of the ligation mixture was used to transform competent TG2 cells.
DH5a E. coli cells were transformed with the ligation mixture.
An aliquot of the ligation mixture is used to transform competent TG2 cells.
JM101 cells were then transformed with the ligation mixture.
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