Examples of 'ligations' in a sentence
Meaning of "ligations"
Ligation refers to the surgical process of tying off blood vessels or ducts, often done to stop bleeding or prevent leakage. It can also refer to the molecular biology technique of joining DNA fragments by using DNA ligase enzyme
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- plural of ligation
How to use "ligations" in a sentence
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ligations
Ligations are preferably conducted by means of a ligase enzyme.
As expected no transformants were obtained in the control ligations.
All ligations are carried out as described above.
The women had previously had tubal ligations.
Tubal ligations are the most common form of sterilization.
This is therefore a construction with two successive ligations.
Some tubal ligations are performed immediately following childbirth.
A small incision is made between the two ligations.
The annealing and ligations were done in two separate reactions.
Insert fragments were diluted appropriately for efficient ligations.
Digestions and ligations were monitored by agarose gel electrophoresis.
These reaction conditions also include chemical ligations.
Six ligations are thereby made.
The dilute plasmid concentration favors intramolecular ligations.
Ligations were carried out as follows.
See also
The ligation was positioned using an adaptor for elastomeric ligations.
Ligations and transformations were carried out according to kit instructions.
This again reduces the number of nonproductive ligations occurring.
Procedure for ligations and transformations are well known in the art.
We have thendeveloped synthetic methodologies combining several orthogonal chemoselective ligations.
Ligations to expression vector.
Appropriately large volumes may also be used to avoid intermolecular ligations.
Set up ligations using a constant amount of vector and increasing amounts of cDNAs.
The adapters are also fixed to a substrate to prevent ligations between these adapters.
Transformants from these ligations were grown up and plasmid DNAs isolated and sequenced.
This is especially true when the user has to perform several consecutive ligations.
To determine the optimum ratio of cDNA to vector several ligations were assembled and electroporated.
Ligations are biocompatible chemical reactions that link two synthetic or biological entities one antother.
Women are also given hysterectomies and tubal ligations at the discretion of the medical officers.
Various known cleavable linkers can be used to monitor the solid phase sequential ligations.
The adapters are also treated with phosphatase so that ligations between these adapters are reduced.
Sticky end ligations require less ATP and less ligase than blunt end ligations.
The restriction sites are contained on the primers for ligations of the PCR fragments.
Ligations are carried out as exemplified by that used in M segment ligations.
Analysis of Clones Colonies from the ligations were first screened for inserts by PCR screening.
Ligations are incubated at room temperature for 2 hours.
The products of such ligations are double-stranded.
Weighted-average assumptions to determine benefit ob ligations.
Ligations were performed overnight at room temperature in a total volume of 10 µl.
Intermolecular blunt end ligations are performed at 1 µM total ends concentration.
As a rule, we perform peripheral ligations.
Intermolecular blunt end ligations are performed at 1 mM total ends concentration.
Ligations to other sequences are performed using standard procedures, known in the art.
This plasmid was constructed by successive ligations of the following elements,.
Optionally, all ligations extend the ligation product in the same direction.
There is no contingent liability set aside for ligations that are each above €2 million.
Therapeutic ligations are held for hepatic neoplasms, trauma and liver arterial lesions.
For example, all ligation reactions can be forward ligations or all can be reverse ligations.
The ligations were transformed into XL1-blue and chloramphenicol resistant colonies were selected.
In other embodiments, some ligations can be forward and some reverse.
