Examples of 'ligation reactions' in a sentence
Meaning of "ligation reactions"
ligation reactions - In the context of molecular biology and genetics, ligation reactions refer to the process of joining DNA fragments together using DNA ligase enzyme. This process is commonly used in genetic engineering, cloning, and other molecular biology techniques
How to use "ligation reactions" in a sentence
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ligation reactions
Ligation reactions for the formation of multimers.
Restriction digest and ligation reactions to clone the variable regions.
The use of better thioester leaving groups resulted in faster ligation reactions.
The following ligation reactions are of the same type.
The invention makes it possible to produce polypeptides by successively carrying out several ligation reactions.
The ligation reactions were incubated at room temperature overnight.
These conditions were used for all the ligation reactions described hereafter.
The ligation reactions were packaged.
The DNA was then digested with NotI and purified prior to use in ligation reactions.
Centrifuge vials containing the ligation reactions briefly and place them on ice.
Ligation reactions produce SBU conjugates with additional nucleic acid sequences appended.
For this assemble ligation reactions composed of,.
The ligation reactions will preferably be enzyme-catalysed.
Coli DH5a electrocompetent cells were transformed with the ligation reactions.
A portion of the ligation reactions was used to transform E.
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In a preferred method of preparation, the hydrogel is generated through chemical ligation reactions.
Prepare ligation reactions in a microcentrifuge tube on ice,.
For both the light and heavy chain transformations, 2 microliters of ligation reactions were used.
Alternatively, ligation reactions can be used.
Ligation reactions were as a standard performed in the following reaction mixtures,.
Prepare the following ligation reactions in microcentrifuge tubes on ice,.
Ligation reactions are used to synthesise combinatorial libraries, label biomolecules and perform supramolecular synthesis.
A variety of template-driven ligation reactions are described in the following references.
This illustrates how oligonucleotides with different tags can take part in sequence-specific ligation reactions.
Package the ligation reactions following manufacturer 's protocol.
In particular examples, at least five different or at least 20 different ligation reactions are performed.
In these ligation reactions about 1 nanomole of each oligonucleotide was used.
Mature tRNA is isolated from both ligation reactions and digested with RNAse T1.
The ligation reactions were purified by phenol / chloroform extraction as described above.
Combine gel fragments from the 7 ligation reactions in two microcentrifuge tubes.
The ligation reactions were made up to 2M ammonium acetate and ethanol precipitated.
Thus, the total amount of amplified mutagenized heavy chain for five ligation reactions was 3.2 µg.
In a next step, separate ligation reactions are performed to combine two segments of interest.
Commercially available competent bacterial strain TOP10 F ' were transformed with the ligation reactions.
For example, all ligation reactions can be forward ligations or all can be reverse ligations.
Various combinations of purified oligos 1-28 were then subject to ligation reactions.
Advantageously, the ligation reactions have fast reaction rates and produce ligation products in high yield.
Coli BL21 ( DE3 ) electrocompetent cells were transformed with the ligation reactions.
Exemplary ligation reactions are described in Example 2.
Fragments were used immediately in ligation reactions or frozen at 20°C for subsequent use.
Ligation reactions were performed using T4 ligase according to the suppliers ' instructions.
Ligation 2, FW3b → FW3a Prepare the following ligation reactions in microcentrifuge tubes on ice,.
The ligation reactions are performed using T4 DNA ligase in the following reaction mixture,.
These ligation reactions were then used to transform competent E. coli strain FM5.
Ligation reactions were used to transform E. coli DH5a.
Ligation reactions were used to transform E. coli DH5a to kanamycin resistance.
The ligation reactions were purified and used to transform competent E. coli DH10B.
The ligation reactions contained equimolar ( 0.6 µM ) concen-trations of template, primer and each of the pentamers.
The ligation reactions were incubated at 16°C overnight.
The ligation reactions ( approximately 250 ng DNA / transformation ) were electroporated into electrocompetent E.
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Examples of using Ligation
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Tubal ligation was performed as previously informed consented
Another method involves ligation vía cohesive ends
Tubal ligation is not just birth control
Examples of using Reactions
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Allergic reactions can occur in different ways
It is quite clear that your reactions are automatic
Chemical reactions involve change on two levels
