Examples of 'ligation reaction' in a sentence
Meaning of "ligation reaction"
Ligation reaction: a molecular biology technique used to join DNA fragments together, typically in the creation of recombinant DNA molecules
How to use "ligation reaction" in a sentence
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ligation reaction
Transform ligation reaction into a competent strain.
The second reaction is a ligation reaction.
A ligation reaction involving three partners has been performed.
Escherichia coli DHa was transformed with the ligation reaction.
One exemplary ligation reaction was carried out as follows.
The number of transformants from each ligation reaction was measured.
Ligation reaction of the gene to pcDNA.
Where two arrows converge is where a ligation reaction is performed.
The ligation reaction is then digested with EcoRI.
Another means of appending additional sequence may be that of a ligation reaction.
Ligation reaction volume can be varied.
A partially degenerate reporter oligonucleotide may be used in the ligation reaction.
A probe ligation reaction may also be used to quantify miRNAs.
A single adapter or two different adapters may be used in a ligation reaction with templates.
The ligation reaction was terminated by phenol extraction followed by ethanol precipitation.
See also
Recombinant plasmids resulting from this ligation reaction were analyzed by restriction analysis.
This ligation reaction is exactly the same as that described in the previous section.
The restriction enzyme treatment reaction conditions and ligation reaction conditions were as follows.
Before adding ligation reaction components mixture was moved to cooling stand for two min.
The elevated reaction temperatures permit the ligation reaction to be conducted with high stringency.
The ligation reaction can be set up using well known techniques and conventional reagents.
Removal of the thiol auxiliary group is preferably performed after completion of the ligation reaction.
The specificity of the ligation reaction was tested using a reaction without ligase.
The metal ion contributes directly or indirectly to a catalysis of the click chemistry ligation reaction.
The ligation reaction was possible because BglII and BamHI ends are compatible with each other.
A number of these were picked from each ligation reaction and used to prepare plasmid DNA.
The ligation reaction is usually performed using a DNA ligase enzyme.
The digested DNA was used in a DNA ligation reaction to recircularize the plasmid.
The ligation reaction was performed by using a ligation kit produced by Takara Shuzo.
A DNA fragment and a DNA segment can be combined though conducting a ligation reaction.
The ligation reaction can be performed by any of a variety of suitable thermostable DNA ligases.
After digestion, the ligation reaction was performed.
The ligation reaction conditions were as follows, ng.
Coli DH5a was transformed by using a part of the ligation reaction mixture.
Five µl of the ligation reaction was subsequently transformed E.
Typically, two binding regions are joined enzymatically by a ligase in a ligation reaction.
Standard ligation reaction conditions are as described by New England Biolabs.
The final concentration of primers in the following ligation reaction was 5µM.
Prepare ligation reaction in a microcentrifuge tube on ice,.
Wherein said method comprises at least one step of ligation reaction between a polypeptide of formula,.
Then, a ligation reaction and transformation were performed.
Following the digestion with the secondary restriction enzyme, a further ligation reaction is performed.
A large-scale ligation reaction was performed as follows.
Figure 5A is a schematic of the enzymatic ligation reaction.
Add 2 µl of the ligation reaction mixture to one aliquot of cells.
However, DNP is gradually removed by thiols during the ligation reaction.
The ligation reaction was performed according to the manufacturer 's instructions.
All expression / secretion vectors were assembled by a similar ligation reaction containing three component fragments.
Two ml of the ligation reaction was used to transform competent InvaF ' cells.
The copper ( I ) contributes to a catalysis of the click chemistry ligation reaction.
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Tubal ligation was performed as previously informed consented
Another method involves ligation vía cohesive ends
Tubal ligation is not just birth control
Examples of using Reaction
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It would give its reaction at a later date
Quick reaction times in case of delay s or malfunctioning
I am having a physical reaction to how bad this is
